Effects of matrix metalloproteinase-1 on the myogenic differentiation of bone marrow-derived mesenchymal stem cells in vitro

Zhenyang Zheng; Yan Leng; Chen Zhou; Zhenyu Ma; Zhigang Zhong; Xing Ming Shi; Weixi Zhang

doi:10.1016/j.bbrc.2012.10.053

Effects of matrix metalloproteinase-1 on the myogenic differentiation of bone marrow-derived mesenchymal stem cells in vitro

Zhenyang Zheng, Yan Leng, Chen Zhou, Zhenyu Ma, Zhigang Zhong, Xing Ming Shi, Weixi Zhang

Neuroscience and Regenerative Medicine

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Matrix metalloproteinase-1 (MMP-1) is a member of the family of zinc-dependent endopeptidases that are capable of degrading extracellular matrix (ECM) and certain non-matrix proteins. It has been shown that MMP-1 can enhance muscle regeneration by improving the differentiation and migration of myoblasts. However, it is still not known whether MMP-1 can promote the myogenesis of bone marrow-derived mesenchymal stem cells (BMSCs). To address this question, we isolated BMSCs from C57BL/6J mice and investigated the effects of MMP-1 on their proliferation and myogenic differentiation. Our results showed that MMP-1 treatment, which had no cytotoxic effects on BMSCs, increased the mRNA and protein levels of MyoD and desmin in a dose-dependent manner, indicating that MMP-1 promoted myogenic differentiation of BMSCs in vitro. These results suggest that BMSCs may have a therapeutic potential for treating muscular disorders.

Original language	English (US)
Pages (from-to)	309-314
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	428
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2012.10.053
State	Published - Nov 16 2012

Keywords

Bone marrow-derived mesenchymal stem cells
Matrix metalloproteinase-1
Myogenesis

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2012.10.053

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title = "Effects of matrix metalloproteinase-1 on the myogenic differentiation of bone marrow-derived mesenchymal stem cells in vitro",

abstract = "Matrix metalloproteinase-1 (MMP-1) is a member of the family of zinc-dependent endopeptidases that are capable of degrading extracellular matrix (ECM) and certain non-matrix proteins. It has been shown that MMP-1 can enhance muscle regeneration by improving the differentiation and migration of myoblasts. However, it is still not known whether MMP-1 can promote the myogenesis of bone marrow-derived mesenchymal stem cells (BMSCs). To address this question, we isolated BMSCs from C57BL/6J mice and investigated the effects of MMP-1 on their proliferation and myogenic differentiation. Our results showed that MMP-1 treatment, which had no cytotoxic effects on BMSCs, increased the mRNA and protein levels of MyoD and desmin in a dose-dependent manner, indicating that MMP-1 promoted myogenic differentiation of BMSCs in vitro. These results suggest that BMSCs may have a therapeutic potential for treating muscular disorders.",

keywords = "Bone marrow-derived mesenchymal stem cells, Matrix metalloproteinase-1, Myogenesis",

author = "Zhenyang Zheng and Yan Leng and Chen Zhou and Zhenyu Ma and Zhigang Zhong and Shi, {Xing Ming} and Weixi Zhang",

note = "Funding Information: We thank Lei Wei and Dingbang Chen for their excellent technical assistance. This study was supported by the National Natural Science Foundation of China (Grant Nos. 30971026 and 30870852 ).",

year = "2012",

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doi = "10.1016/j.bbrc.2012.10.053",

language = "English (US)",

volume = "428",

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T1 - Effects of matrix metalloproteinase-1 on the myogenic differentiation of bone marrow-derived mesenchymal stem cells in vitro

AU - Zheng, Zhenyang

AU - Leng, Yan

AU - Zhou, Chen

AU - Ma, Zhenyu

AU - Zhong, Zhigang

AU - Shi, Xing Ming

AU - Zhang, Weixi

N1 - Funding Information: We thank Lei Wei and Dingbang Chen for their excellent technical assistance. This study was supported by the National Natural Science Foundation of China (Grant Nos. 30971026 and 30870852 ).

PY - 2012/11/16

Y1 - 2012/11/16

N2 - Matrix metalloproteinase-1 (MMP-1) is a member of the family of zinc-dependent endopeptidases that are capable of degrading extracellular matrix (ECM) and certain non-matrix proteins. It has been shown that MMP-1 can enhance muscle regeneration by improving the differentiation and migration of myoblasts. However, it is still not known whether MMP-1 can promote the myogenesis of bone marrow-derived mesenchymal stem cells (BMSCs). To address this question, we isolated BMSCs from C57BL/6J mice and investigated the effects of MMP-1 on their proliferation and myogenic differentiation. Our results showed that MMP-1 treatment, which had no cytotoxic effects on BMSCs, increased the mRNA and protein levels of MyoD and desmin in a dose-dependent manner, indicating that MMP-1 promoted myogenic differentiation of BMSCs in vitro. These results suggest that BMSCs may have a therapeutic potential for treating muscular disorders.

AB - Matrix metalloproteinase-1 (MMP-1) is a member of the family of zinc-dependent endopeptidases that are capable of degrading extracellular matrix (ECM) and certain non-matrix proteins. It has been shown that MMP-1 can enhance muscle regeneration by improving the differentiation and migration of myoblasts. However, it is still not known whether MMP-1 can promote the myogenesis of bone marrow-derived mesenchymal stem cells (BMSCs). To address this question, we isolated BMSCs from C57BL/6J mice and investigated the effects of MMP-1 on their proliferation and myogenic differentiation. Our results showed that MMP-1 treatment, which had no cytotoxic effects on BMSCs, increased the mRNA and protein levels of MyoD and desmin in a dose-dependent manner, indicating that MMP-1 promoted myogenic differentiation of BMSCs in vitro. These results suggest that BMSCs may have a therapeutic potential for treating muscular disorders.

KW - Bone marrow-derived mesenchymal stem cells

KW - Matrix metalloproteinase-1

KW - Myogenesis

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Effects of matrix metalloproteinase-1 on the myogenic differentiation of bone marrow-derived mesenchymal stem cells in vitro

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