Effects of streptozotocin- and alloxan-induced diabetes mellitus on mouse follicular and early embryo development

Michael Peter Diamond, K. H. Moley, A. Pellicer, W. K. Vaughn, A. H. DeCherney

Research output: Contribution to journalArticlepeer-review

107 Scopus citations


Mice were made diabetic by intraperitoneal injection of streptozotocin or alloxan. Germinal vesicle breakdown in the ovarian follicles at 8 h after hCG in control animals (57%) was significantly greater than in streptozotocin-(24%) and alloxan-(42%) diabetic animals (P < 0.001). This delay in oocyte maturation was reversible by in-vivo insulin administration to diabetic mice. A developmental delay was also found for embryos recovered from diabetic mice. This developmental delay extended into the 72 h in-vitro cultures. Compared to control embryos, those from alloxan- and streptozotocin-treated mice demonstrated marked impairment in development as assessed by (1) distribution of developmental cell stages at each observation period and (2) rates of development which increasingly diverged at each observation period. In diabetic mice treated with insulin in vivo, the percentage of 2-cell embryos recovered increased. Furthermore, in streptozotocin- and alloxan-animals treated with insulin, the rate of in-vitro development of embryos, as well as their developmental stage distribution improved. We therefore suggest that uncontrolled diabetes mellitus, as well as contributing to the development of congenital malformations, may deleteriously affect reproductive performance both before fertilization and at the very earliest gestational stages.

Original languageEnglish (US)
Pages (from-to)1-10
Number of pages10
JournalJournal of Reproduction and Fertility
Issue number1
StatePublished - 1989

ASJC Scopus subject areas

  • Physiology
  • Embryology
  • Molecular Biology
  • Obstetrics and Gynecology
  • Developmental Biology


Dive into the research topics of 'Effects of streptozotocin- and alloxan-induced diabetes mellitus on mouse follicular and early embryo development'. Together they form a unique fingerprint.

Cite this