Efficacy of Two Contemporary Single-cone Filling Techniques in Preventing Bacterial Leakage

Francesca Monticelli, Fernanda T. Sadek, George S. Schuster, Keith R. Volkmann, Stephen W. Looney, Marco Ferrari, Manuel Toledano, David H. Pashley, Franklin R. Tay

Research output: Contribution to journalArticlepeer-review

48 Scopus citations


This in vitro study evaluated the sealing efficacy of three root-filling systems/techniques in preventing bacterial leakage. Instrumented single-rooted root segments were filled with (1) warm vertical compaction with gutta-percha/AH Plus; (2) single-cone technique with ActiV GP; and (3) single-cone technique with Gutta-Flow. A dual-chamber leakage model using S. mutans as a microbial marker was used for leakage evaluation. Bacterial penetration was monitored over a 100-day period. Leakage was recorded when turbidity was observed in the lower chamber. Gutta-percha warm vertical compaction exhibited the best seal with bacterial leakage observed in only 16.7% of the specimens between 59 and 100 days. All ActiV GP specimens leaked between 7 and 100 days; 50% of the Gutta-Flow specimens leaked between 22 and 100 days. The two contemporary single-cone techniques did not insure a durable apical seal against bacterial leakage. A warm vertical compaction technique using thermoplasticized gutta-percha and AH Plus sealer appears to be more effective in minimizing bacterial leakage.

Original languageEnglish (US)
Pages (from-to)310-313
Number of pages4
JournalJournal of endodontics
Issue number3
StatePublished - Mar 2007


  • AH Plus
  • ActiV GP
  • Gutta-Flow
  • bacterial leakage
  • coronal seal
  • single-cone

ASJC Scopus subject areas

  • Dentistry(all)


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