TY - JOUR
T1 - Electronic imaging system for direct and rapid quantitation of fluorescence from electrophoretic gels
T2 - Application to ethidium bromide-stained DNA
AU - Sutherland, John Clark
AU - Lin, Bohai
AU - Monteleone, Denise C.
AU - Mugavero, Jo Ann
AU - Sutherland, Betsy M.
AU - Trunk, John
N1 - Funding Information:
’ This research was sponsored by the Office of Health and Environmental Research, U.S. Department of Energy and by a grant from the National Institutes of Health (GM-35662) to J.C.S. ’ To whom correspondence should be addressed. 3 Present address: Institute of Biophysics, Academia Sinica, Bejing, China.
PY - 1987/6
Y1 - 1987/6
N2 - We have built an electronic imaging system based on a modified charge-coupled-device television camera that directly quantitates the distribution of fluorescence from electrophoretic gels, chromatograms, and other stationary sources. Exposure times can exceed 1 min. Unlike the photographic system that it replaces, the response of the camera is directly proportional to the intensity of incident fluorescence, and image data are digitized and stored in computer memory ready for analysis immediately upon completion of an exposure. We describe procedures for the display, normalization, and archival storage of image data and programs that use images of ethidium bromide-stained DNA in alkaline agarose gels to quantitate single-strand breaks in DNA.
AB - We have built an electronic imaging system based on a modified charge-coupled-device television camera that directly quantitates the distribution of fluorescence from electrophoretic gels, chromatograms, and other stationary sources. Exposure times can exceed 1 min. Unlike the photographic system that it replaces, the response of the camera is directly proportional to the intensity of incident fluorescence, and image data are digitized and stored in computer memory ready for analysis immediately upon completion of an exposure. We describe procedures for the display, normalization, and archival storage of image data and programs that use images of ethidium bromide-stained DNA in alkaline agarose gels to quantitate single-strand breaks in DNA.
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U2 - 10.1016/0003-2697(87)90247-8
DO - 10.1016/0003-2697(87)90247-8
M3 - Article
C2 - 3661993
AN - SCOPUS:0023355323
SN - 0003-2697
VL - 163
SP - 446
EP - 457
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -