EMR-3: A potential mediator of invasive phenotypic variation in glioblastoma and novel therapeutic target

Ari J. Kane; Michael E. Sughrue; Martin J. Rutkowski; Joanna J. Phillips; Andrew T. Parsa

doi:10.1097/WNR.0b013e32833f19f2

EMR-3: A potential mediator of invasive phenotypic variation in glioblastoma and novel therapeutic target

Ari J. Kane, Michael E. Sughrue, Martin J. Rutkowski, Joanna J. Phillips, Andrew T. Parsa

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Epidermal growth factor module-containing mucin-like hormone receptor-3 (EMR-3) is a G-protein coupled receptor with unknown ligand and cellular function. Upregulation of EMR-3 in glioblastoma (GBM) multiforme is associated with poor survival. We investigated the expression patterns and functional significance of EMR-3 in GBM using immunohistochemistry, western blot, reverse transcription PCR, and small interfering RNA knockdown in proliferation and invasion assays. EMR-3 is variably expressed in primary human GBM tissues and cell lines. Knocking down EMR-3 has no impact on cellular proliferation, but decreases cellular invasion by greater than 3-fold. EMR-3 is a potential mediator of cellular invasion in GBM. Given the poor survival associated with high levels of EMR-3 expression in glioma patients, our results provide impetus to explore EMR-3 as a potential therapeutic target.

Original language	English (US)
Pages (from-to)	1018-1022
Number of pages	5
Journal	NeuroReport
Volume	21
Issue number	16
DOIs	https://doi.org/10.1097/WNR.0b013e32833f19f2
State	Published - Nov 17 2010
Externally published	Yes

Keywords

EGF module containing mucin-like hormone receptor-3
glioma
invasion

ASJC Scopus subject areas

Neuroscience(all)

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10.1097/WNR.0b013e32833f19f2

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title = "EMR-3: A potential mediator of invasive phenotypic variation in glioblastoma and novel therapeutic target",

abstract = "Epidermal growth factor module-containing mucin-like hormone receptor-3 (EMR-3) is a G-protein coupled receptor with unknown ligand and cellular function. Upregulation of EMR-3 in glioblastoma (GBM) multiforme is associated with poor survival. We investigated the expression patterns and functional significance of EMR-3 in GBM using immunohistochemistry, western blot, reverse transcription PCR, and small interfering RNA knockdown in proliferation and invasion assays. EMR-3 is variably expressed in primary human GBM tissues and cell lines. Knocking down EMR-3 has no impact on cellular proliferation, but decreases cellular invasion by greater than 3-fold. EMR-3 is a potential mediator of cellular invasion in GBM. Given the poor survival associated with high levels of EMR-3 expression in glioma patients, our results provide impetus to explore EMR-3 as a potential therapeutic target.",

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T2 - A potential mediator of invasive phenotypic variation in glioblastoma and novel therapeutic target

AU - Kane, Ari J.

AU - Sughrue, Michael E.

AU - Rutkowski, Martin J.

AU - Phillips, Joanna J.

AU - Parsa, Andrew T.

PY - 2010/11/17

Y1 - 2010/11/17

N2 - Epidermal growth factor module-containing mucin-like hormone receptor-3 (EMR-3) is a G-protein coupled receptor with unknown ligand and cellular function. Upregulation of EMR-3 in glioblastoma (GBM) multiforme is associated with poor survival. We investigated the expression patterns and functional significance of EMR-3 in GBM using immunohistochemistry, western blot, reverse transcription PCR, and small interfering RNA knockdown in proliferation and invasion assays. EMR-3 is variably expressed in primary human GBM tissues and cell lines. Knocking down EMR-3 has no impact on cellular proliferation, but decreases cellular invasion by greater than 3-fold. EMR-3 is a potential mediator of cellular invasion in GBM. Given the poor survival associated with high levels of EMR-3 expression in glioma patients, our results provide impetus to explore EMR-3 as a potential therapeutic target.

AB - Epidermal growth factor module-containing mucin-like hormone receptor-3 (EMR-3) is a G-protein coupled receptor with unknown ligand and cellular function. Upregulation of EMR-3 in glioblastoma (GBM) multiforme is associated with poor survival. We investigated the expression patterns and functional significance of EMR-3 in GBM using immunohistochemistry, western blot, reverse transcription PCR, and small interfering RNA knockdown in proliferation and invasion assays. EMR-3 is variably expressed in primary human GBM tissues and cell lines. Knocking down EMR-3 has no impact on cellular proliferation, but decreases cellular invasion by greater than 3-fold. EMR-3 is a potential mediator of cellular invasion in GBM. Given the poor survival associated with high levels of EMR-3 expression in glioma patients, our results provide impetus to explore EMR-3 as a potential therapeutic target.

KW - EGF module containing mucin-like hormone receptor-3

KW - glioma

KW - invasion

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EMR-3: A potential mediator of invasive phenotypic variation in glioblastoma and novel therapeutic target

Abstract

Keywords

ASJC Scopus subject areas

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