TY - JOUR
T1 - Enamel remineralization via poly(amido amine) and adhesive resin containing calcium phosphate nanoparticles
AU - Gao, Yuan
AU - Liang, Kunneng
AU - Weir, Michael D.
AU - Gao, Jianghong
AU - Imazato, Satoshi
AU - Tay, Franklin R.
AU - Lynch, Christopher D.
AU - Oates, Thomas W.
AU - Li, Jiyao
AU - Xu, Hockin H.K.
N1 - Funding Information:
This work was supported by National Natural Science Foundation of China81670977 (L.J.Y.) and 81800965 (L.K.N.), Sichuan Science and Technology program (Grant No. 2017SZ0030), the Fundamental Research Funds for the Central University2018SCU12016 (L.K.N.), the Research Fund of West China Hospital<GN4>WCHS-201705</GN4> (L.K.N.) and the China Postdoctoral Foundation2018M643507 (L.K.N.), a seed grant from the University of Maryland Baltimore (HX), and a bridge grant from the University of Maryland School of Dentistry (HX).
Funding Information:
This work was supported by National Natural Science Foundation of China 81670977 (L.J.Y.) and 81800965 (L.K.N.), Sichuan Science and Technology program (Grant No. 2017SZ0030 ), the Fundamental Research Funds for the Central University 2018SCU12016 (L.K.N.), the Research Fund of West China Hospital WCHS- 201705 (L.K.N.) and the China Postdoctoral Foundation 2018M643507 (L.K.N.), a seed grant from the University of Maryland Baltimore (HX), and a bridge grant from the University of Maryland School of Dentistry (HX).
Publisher Copyright:
© 2019 Elsevier Ltd
PY - 2020/1
Y1 - 2020/1
N2 - Objectives: The objective of this study was to investigate enamel remineralization using salivary statherin protein-inspired poly(amidoamine) dendrimer (SN15-PAMAM) and adhesive containing nanoparticles of amorphous calcium phosphate (NACP) in a cyclic artificial saliva/demineralizing solution for the first time. Methods: The enamel shear bond strengths of NACP adhesives were measured compared to commercial adhesive (Scotchbond Multi-Purpose, 3 M). Adhesive disks containing NACP were tested for calcium (Ca) and phosphorus (P) ions release. Four groups were tested: (1) enamel control, (2) enamel with NACP, (3) enamel with SN15-PAMAM, and (4) enamel with SN15-PAMAM + NACP. The specimens were treated with cyclic artificial saliva/demineralizing solution for 28 days. The remineralized enamel specimens were examined by surface and cross-sectional hardness test. Results: NACP adhesive yielded a similar shear bond strength to commercial control (Scotchbond Multi-Purpose, 3 M). NACP adhesive released high levels of Ca and P ions. At 28 days, the enamel hardness of SN15-PAMAM + NACP group was 2.89 ± 0.13 GPa, significantly higher than that of control group (1.46 ± 0.10 GPa) (p < 0.05). SN15-PAMAM + NACP increased the enamel cross-sectional hardness at 28 days; at 25 μm, enamel cross-sectional hardness was 90 % higher than that of control group (p < 0.05). Significance: The novel SN15-PAMAM + NACP adhesive method could achieve 90 % higher enamel remineralization of the artificial caries than the control under acid challenge for the first time. This method is promising for use after tooth cavity preparation, or as a coating on enamel with white spot lesions (WSLs) for prevention, to reduce secondary caries, prevent caries procession and protect tooth structures.
AB - Objectives: The objective of this study was to investigate enamel remineralization using salivary statherin protein-inspired poly(amidoamine) dendrimer (SN15-PAMAM) and adhesive containing nanoparticles of amorphous calcium phosphate (NACP) in a cyclic artificial saliva/demineralizing solution for the first time. Methods: The enamel shear bond strengths of NACP adhesives were measured compared to commercial adhesive (Scotchbond Multi-Purpose, 3 M). Adhesive disks containing NACP were tested for calcium (Ca) and phosphorus (P) ions release. Four groups were tested: (1) enamel control, (2) enamel with NACP, (3) enamel with SN15-PAMAM, and (4) enamel with SN15-PAMAM + NACP. The specimens were treated with cyclic artificial saliva/demineralizing solution for 28 days. The remineralized enamel specimens were examined by surface and cross-sectional hardness test. Results: NACP adhesive yielded a similar shear bond strength to commercial control (Scotchbond Multi-Purpose, 3 M). NACP adhesive released high levels of Ca and P ions. At 28 days, the enamel hardness of SN15-PAMAM + NACP group was 2.89 ± 0.13 GPa, significantly higher than that of control group (1.46 ± 0.10 GPa) (p < 0.05). SN15-PAMAM + NACP increased the enamel cross-sectional hardness at 28 days; at 25 μm, enamel cross-sectional hardness was 90 % higher than that of control group (p < 0.05). Significance: The novel SN15-PAMAM + NACP adhesive method could achieve 90 % higher enamel remineralization of the artificial caries than the control under acid challenge for the first time. This method is promising for use after tooth cavity preparation, or as a coating on enamel with white spot lesions (WSLs) for prevention, to reduce secondary caries, prevent caries procession and protect tooth structures.
KW - Adhesive
KW - Enamel hardness
KW - Enamel remineralization
KW - Nano-calcium phosphate
KW - Statherin protein poly(amidoamine) dendrimer
KW - pH cycling
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U2 - 10.1016/j.jdent.2019.103262
DO - 10.1016/j.jdent.2019.103262
M3 - Article
C2 - 31837358
AN - SCOPUS:85076998850
SN - 0300-5712
VL - 92
JO - Journal of Dentistry
JF - Journal of Dentistry
M1 - 103262
ER -