TY - JOUR
T1 - Enumeration of single IFN-γ-producing cells in mice during viral and bacterial infection
AU - Gessner, A.
AU - Moskofidis, Dimitrios
AU - Lehmann-Grube, F.
PY - 1989/1/1
Y1 - 1989/1/1
N2 - A solid phas immunoenzymatic technique was employed for detecting single IFN-γ-producing cells (IFN-γPC) in the mouse. After infection with lymphocytic choriomeningitis virus or Listeria monocytogenes, the numbers of IFN-γPC in spleens began to rise on day 4, attained maxima on days 7 and 8, and declined thereafter. Negative selection in vitro by use of mAb and C allowed phenotypic identification of the producer cells; most, if not all, carried Thy-1, and approximately one half expressed CD4, the other half, CD8. Depletion of cells in vivo by treatment of mice with mAb led to somewhat different results; again, anti-Thy-1 antibody eliminated essentially all IFN-γPC, but considerably more than 50% were either CD4+ or CD8+, suggesting regulatory interactions between these T lymphocyte subsets with regard to generation of the lymphokine.
AB - A solid phas immunoenzymatic technique was employed for detecting single IFN-γ-producing cells (IFN-γPC) in the mouse. After infection with lymphocytic choriomeningitis virus or Listeria monocytogenes, the numbers of IFN-γPC in spleens began to rise on day 4, attained maxima on days 7 and 8, and declined thereafter. Negative selection in vitro by use of mAb and C allowed phenotypic identification of the producer cells; most, if not all, carried Thy-1, and approximately one half expressed CD4, the other half, CD8. Depletion of cells in vivo by treatment of mice with mAb led to somewhat different results; again, anti-Thy-1 antibody eliminated essentially all IFN-γPC, but considerably more than 50% were either CD4+ or CD8+, suggesting regulatory interactions between these T lymphocyte subsets with regard to generation of the lymphokine.
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M3 - Article
C2 - 2492578
AN - SCOPUS:0024561556
SN - 0022-1767
VL - 142
SP - 1293
EP - 1298
JO - Journal of Immunology
JF - Journal of Immunology
IS - 4
ER -