TY - JOUR
T1 - Ependymal cell contribution to scar formation after spinal cord injury is minimal, local and dependent on direct ependymal injury
AU - Ren, Yilong
AU - Ao, Yan
AU - O'Shea, Timothy M.
AU - Burda, Joshua E.
AU - Bernstein, Alexander M.
AU - Brumm, Andrew J.
AU - Muthusamy, Nagendran
AU - Ghashghaei, H. Troy
AU - Carmichael, S. Thomas
AU - Cheng, Liming
AU - Sofroniew, Michael V.
N1 - Publisher Copyright:
© The Author(s) 2017.
PY - 2017/1/24
Y1 - 2017/1/24
N2 - Ependyma have been proposed as adult neural stem cells that provide the majority of newly proliferated scar-forming astrocytes that protect tissue and function after spinal cord injury (SCI). This proposal was based on small, midline stab SCI. Here, we tested the generality of this proposal by using a genetic knock-in cell fate mapping strategy in different murine SCI models. After large crush injuries across the entire spinal cord, ependyma-derived progeny remained local, did not migrate and contributed few cells of any kind and less than 2%, if any, of the total newly proliferated and molecularly confirmed scar-forming astrocytes. Stab injuries that were near to but did not directly damage ependyma, contained no ependyma-derived cells. Our findings show that ependymal contribution of progeny after SCI is minimal, local and dependent on direct ependymal injury, indicating that ependyma are not a major source of endogenous neural stem cells or neuroprotective astrocytes after SCI.
AB - Ependyma have been proposed as adult neural stem cells that provide the majority of newly proliferated scar-forming astrocytes that protect tissue and function after spinal cord injury (SCI). This proposal was based on small, midline stab SCI. Here, we tested the generality of this proposal by using a genetic knock-in cell fate mapping strategy in different murine SCI models. After large crush injuries across the entire spinal cord, ependyma-derived progeny remained local, did not migrate and contributed few cells of any kind and less than 2%, if any, of the total newly proliferated and molecularly confirmed scar-forming astrocytes. Stab injuries that were near to but did not directly damage ependyma, contained no ependyma-derived cells. Our findings show that ependymal contribution of progeny after SCI is minimal, local and dependent on direct ependymal injury, indicating that ependyma are not a major source of endogenous neural stem cells or neuroprotective astrocytes after SCI.
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U2 - 10.1038/srep41122
DO - 10.1038/srep41122
M3 - Article
C2 - 28117356
AN - SCOPUS:85010875151
SN - 2045-2322
VL - 7
JO - Scientific reports
JF - Scientific reports
M1 - 41122
ER -