Epidermal growth factor and cyclic AMP stimulation of distinct protein kinase activities in Leydig cell tumor membranes

Michael H. Melner; William A. Lutin; David Puett

doi:10.1016/0024-3205(82)90437-4

Epidermal growth factor and cyclic AMP stimulation of distinct protein kinase activities in Leydig cell tumor membranes

Michael H. Melner, William A. Lutin, David Puett

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3 Scopus citations

Abstract

Epidermal growth factor (EGF) and cyclic AMP were found to stimulate distinct protein kinase activities in plasma membranes prepared from the M5480P murine Leydig cell tumor. EGF stimulated the phosphorylation of two protein bands with apparent molecular weights of 60,000 and 180,000, while cyclic AMP stimulated the phosphorylation of a minor component of molecular weight 220,000. The two types of kinases could also be distinguished on the basis of differential susceptibility to conditions of membrane preparation. These results suggest that EGF stimulates a cyclic AMP-independent protein kinase in murine Leydig cell tumors at the level of the plasma membrane.

Original language	English (US)
Pages (from-to)	1981-1986
Number of pages	6
Journal	Life sciences
Volume	30
Issue number	23
DOIs	https://doi.org/10.1016/0024-3205(82)90437-4
State	Published - Jun 7 1982
Externally published	Yes

ASJC Scopus subject areas

General Pharmacology, Toxicology and Pharmaceutics
General Biochemistry, Genetics and Molecular Biology

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10.1016/0024-3205(82)90437-4

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@article{c69c9ba486924bd89b230b1b2130a5dc,

title = "Epidermal growth factor and cyclic AMP stimulation of distinct protein kinase activities in Leydig cell tumor membranes",

abstract = "Epidermal growth factor (EGF) and cyclic AMP were found to stimulate distinct protein kinase activities in plasma membranes prepared from the M5480P murine Leydig cell tumor. EGF stimulated the phosphorylation of two protein bands with apparent molecular weights of 60,000 and 180,000, while cyclic AMP stimulated the phosphorylation of a minor component of molecular weight 220,000. The two types of kinases could also be distinguished on the basis of differential susceptibility to conditions of membrane preparation. These results suggest that EGF stimulates a cyclic AMP-independent protein kinase in murine Leydig cell tumors at the level of the plasma membrane.",

author = "Melner, {Michael H.} and Lutin, {William A.} and David Puett",

note = "Funding Information: This work was supported by NIH Research Grant AM15838 and Training Grants CA09131 and EY07007. We thank Dr. Graham Carpenter for helpful discussions and for providing facilities under the auspices of NIH Center Grant HD05797. Dr. P. Galen Lenhert is gratefully acknowledged for providing access to the microdensitometer. Lastly, we thank Drs. Stanley Cohen and Lawrence J. Dangott for providing purified mouse EGF and e2-macroglobulin, respectively.",

year = "1982",

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doi = "10.1016/0024-3205(82)90437-4",

language = "English (US)",

volume = "30",

pages = "1981--1986",

journal = "Life sciences",

issn = "0024-3205",

publisher = "Elsevier Inc.",

number = "23",

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TY - JOUR

T1 - Epidermal growth factor and cyclic AMP stimulation of distinct protein kinase activities in Leydig cell tumor membranes

AU - Melner, Michael H.

AU - Lutin, William A.

AU - Puett, David

N1 - Funding Information: This work was supported by NIH Research Grant AM15838 and Training Grants CA09131 and EY07007. We thank Dr. Graham Carpenter for helpful discussions and for providing facilities under the auspices of NIH Center Grant HD05797. Dr. P. Galen Lenhert is gratefully acknowledged for providing access to the microdensitometer. Lastly, we thank Drs. Stanley Cohen and Lawrence J. Dangott for providing purified mouse EGF and e2-macroglobulin, respectively.

PY - 1982/6/7

Y1 - 1982/6/7

N2 - Epidermal growth factor (EGF) and cyclic AMP were found to stimulate distinct protein kinase activities in plasma membranes prepared from the M5480P murine Leydig cell tumor. EGF stimulated the phosphorylation of two protein bands with apparent molecular weights of 60,000 and 180,000, while cyclic AMP stimulated the phosphorylation of a minor component of molecular weight 220,000. The two types of kinases could also be distinguished on the basis of differential susceptibility to conditions of membrane preparation. These results suggest that EGF stimulates a cyclic AMP-independent protein kinase in murine Leydig cell tumors at the level of the plasma membrane.

AB - Epidermal growth factor (EGF) and cyclic AMP were found to stimulate distinct protein kinase activities in plasma membranes prepared from the M5480P murine Leydig cell tumor. EGF stimulated the phosphorylation of two protein bands with apparent molecular weights of 60,000 and 180,000, while cyclic AMP stimulated the phosphorylation of a minor component of molecular weight 220,000. The two types of kinases could also be distinguished on the basis of differential susceptibility to conditions of membrane preparation. These results suggest that EGF stimulates a cyclic AMP-independent protein kinase in murine Leydig cell tumors at the level of the plasma membrane.

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AN - SCOPUS:0019963399

SN - 0024-3205

VL - 30

SP - 1981

EP - 1986

JO - Life sciences

JF - Life sciences

IS - 23

ER -

Epidermal growth factor and cyclic AMP stimulation of distinct protein kinase activities in Leydig cell tumor membranes

Abstract

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