TY - JOUR
T1 - Epitope repertoire of human CD4+ T cells on tetanus toxin
T2 - Identification of immunodominant sequence segments
AU - Diethelm-Okita, B. M.
AU - Raju, R.
AU - Okita, D. K.
AU - Conti-Fine, B. M.
PY - 1997
Y1 - 1997
N2 - Sequence regions of tetanus toxin-forming CD4+ cell epitopes in 8 HLA- disparate subjects were identified. Overlapping synthetic peptides corresponding to the complete tetanus toxin sequence were used to test, in a proliferation assay, unselected blood CD4+ cells or CD4+ cell lines propagated by stimulation with tetanus toxoid. The CD4+ cell lines recognized most peptides recognized by the blood CD4+ cells and they recognized additional peptides. Their responses were stronger than those of unselected blood CD4+ cells. Two peptides were recognized by all subjects: one largely overlapped a tetanus toxin sequence region previously identified as a 'universal' T cell epitope. Thirteen other peptides elicited a CD4+ cell response in 6 or 7 of the 8 subjects, and another 10 elicited responses in 5 subjects.
AB - Sequence regions of tetanus toxin-forming CD4+ cell epitopes in 8 HLA- disparate subjects were identified. Overlapping synthetic peptides corresponding to the complete tetanus toxin sequence were used to test, in a proliferation assay, unselected blood CD4+ cells or CD4+ cell lines propagated by stimulation with tetanus toxoid. The CD4+ cell lines recognized most peptides recognized by the blood CD4+ cells and they recognized additional peptides. Their responses were stronger than those of unselected blood CD4+ cells. Two peptides were recognized by all subjects: one largely overlapped a tetanus toxin sequence region previously identified as a 'universal' T cell epitope. Thirteen other peptides elicited a CD4+ cell response in 6 or 7 of the 8 subjects, and another 10 elicited responses in 5 subjects.
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U2 - 10.1093/infdis/175.2.382
DO - 10.1093/infdis/175.2.382
M3 - Article
C2 - 9203659
AN - SCOPUS:0031033860
SN - 0022-1899
VL - 175
SP - 382
EP - 391
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 2
ER -