TY - JOUR
T1 - Epitopes for human CD4+ cells on diphtheria toxin
T2 - Structural features of sequence segments forming epitopes recognized by most subjects
AU - Raju, Raghavanpillai
AU - Navaneetham, Duraiswamy
AU - Okita, David
AU - Diethelm‐Okita, Brenda
AU - McCormick, Daniel
AU - Conti‐Fine, Bianca M.
PY - 1995/12
Y1 - 1995/12
N2 - The sequence regions of diphtheria toxin (DTX) recognized by CD4+ T cells of seven healthy humans of different major histocompatibility complex haplotypes were identified. Overlapping synthetic peptides, screening the DTX sequence, were used to test in proliferation assays unselected blood CD4+ cells, or DTX‐specific CD4+ lines propagated by stimulation with DTX of blood mononuclear cells. Blood CD4+ cells and DTX‐specific CD4+ lines gave consistent results. Although each subject had an individual pattern of peptide recognition, six peptide sequences (residues 271–290, 321–340, 331–350, 351–370, 411–430 and 431–450) were recognized by all subjects. In the native DTX molecule, these sequence regions are flanked by sequence loops exposed on the DTX surface. They overlap uncharged segments of the DTX sequence. These structural properties may be general requirements for immunodominance in CD4+ cell sensitization in humans.
AB - The sequence regions of diphtheria toxin (DTX) recognized by CD4+ T cells of seven healthy humans of different major histocompatibility complex haplotypes were identified. Overlapping synthetic peptides, screening the DTX sequence, were used to test in proliferation assays unselected blood CD4+ cells, or DTX‐specific CD4+ lines propagated by stimulation with DTX of blood mononuclear cells. Blood CD4+ cells and DTX‐specific CD4+ lines gave consistent results. Although each subject had an individual pattern of peptide recognition, six peptide sequences (residues 271–290, 321–340, 331–350, 351–370, 411–430 and 431–450) were recognized by all subjects. In the native DTX molecule, these sequence regions are flanked by sequence loops exposed on the DTX surface. They overlap uncharged segments of the DTX sequence. These structural properties may be general requirements for immunodominance in CD4+ cell sensitization in humans.
KW - Diphtheria toxin
KW - Universal T helper epitopes
KW - Vaccines
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U2 - 10.1002/eji.1830251202
DO - 10.1002/eji.1830251202
M3 - Article
C2 - 8566002
AN - SCOPUS:0029557484
SN - 0014-2980
VL - 25
SP - 3207
EP - 3214
JO - European Journal of Immunology
JF - European Journal of Immunology
IS - 12
ER -