Evaluation of a Collagen-Reactive Monomer with Advanced Bonding Durability

F. Yu, M. L. Luo, R. C. Xu, L. Huang, W. Zhou, J. Li, F. R. Tay, L. N. Niu, J. H. Chen

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


This study evaluated the use of a new collagen-reactive monomer (CRM), isocyanate-terminated urethane methacrylate precursor, which has covalent affinity to dental collagen, in the formation of dentin-resin bonds and compared it with 2 other dental adhesives. Dentin specimens were bonded with either the CRM-based adhesive (CBA), One-Step (OS; Bisco, Inc.), or a negative adhesive (NA) control and subjected to 24-h storage in water, thermocycling to simulate 1-y clinical function, or a matrix metalloproteinase–mediated aging process. We tested the microtensile bond strength (µTBS), characterized the bonding interface with an atomic force microscope, conducted micro-Raman analysis, and performed leakage tests and in situ zymography. CBA and OS exhibited comparable bonding strength after 24 h (P > 0.05); however, there was a sharp decrease in µTBS after aging for all except CBA (P < 0.001). Raman spectra results indicated increased collagen crosslinking and chemical reaction between the adhesive and collagen in the CBA group. CBA achieved high-quality hybridization with collagen, improving mechanical properties and integrity, and decreased the enzyme-mediated degradation of the bonding interface by inhibiting collagenolytic activity. With the promising bonding durability of coapplied CBA, CRM may be the first dental adhesive to provide strong and long-lasting resin-dental collagen bonding without the additional conditioning step. The use of CBA results in high-quality hybrid layers that protect the resin-dentin interface from harmful biological and chemical activities commonly occurring in the oral environment.

Original languageEnglish (US)
Pages (from-to)813-819
Number of pages7
JournalJournal of Dental Research
Issue number7
StatePublished - Jul 1 2020


  • adhesive
  • dentin
  • hybrid layer
  • isocyanate
  • leakage
  • zymography

ASJC Scopus subject areas

  • General Dentistry


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