TY - JOUR
T1 - Evaluation of two rodent delayed-response memory tasks
T2 - A method with retractable levers versus a method with closing doors
AU - Evans-Martin, F. Fay
AU - Terry, Alvin V.
AU - Jackson, William J.
AU - Buccafusco, Jerry J.
N1 - Funding Information:
This study was supported by the Medical College of Georgia Research Institute and the Medical Research Service of the Department of Veterans Affairs Medical Center. The authors would like to thank Dr. John R. Searle and David M. Parrish of the Department of Biomedical Engineering, Medical College of Georgia, for their contributions to the design of the automated behavioral equipment and related computer software. We also gratefully acknowledge the contribution of Dr. William O. Thompson and Ms. Maribeth Johnson of the Department of Research Computing and Statistics, Medical College of Georgia. Also, we would like to acknowledge Ms. Lisa Erwin and Ms. Bridgett Flanders for technical support.
PY - 2000
Y1 - 2000
N2 - The purpose of this study was to evaluate and compare two similar rodent memory tasks developed in our laboratory that employ stimulus discrimination and delayed response (light and tone stimuli and variable length delays) and to determine their sensitivity to the muscarinic-acetylcholine receptor (mAChR) antagonist, scopolamine hydrobromide (SCOP HBr), and its quaternary (methylbromide) analog (SCOP MBr). Male Wistar rats were trained in either an open chamber that employed retracting levers (RLM) during the delays, or a method that utilized closing doors (CDM) that separated the rats from the levers during delays to reduce positional (nonmnemonic) strategies. When the rats were well trained, dose-effect studies (μg/kg doses, s.c., 30 min before test sessions) of SCOP HBr or MBr were performed. Baseline performance was characterized by delay-dependent decreases in accuracy in both methods except when the tone was the stimulus in the RLM. SCOP HBr impaired performance in both tasks at the higher doses tested, although the effects were more consistent in the CDM task and accuracy associated with each stimulus was affected similarly. Surprisingly, SCOP MBr also impaired performance of each task, especially when the tone was the stimulus, while accuracy associated with the light was not affected in the CDM task. Overall, the results indicated that the CDM was a somewhat more reliable task, appearing to reduce positional strategies with less variability in response to the mAChR antagonists, although some stimulus-modality specific effects were noted. It also appears important to consider the peripheral effects of mAChR antagonists (and potential central effects of quaternary mAChR antagonists) when interpreting results from behavioral studies, especially those involving conditional discrimination and delayed response. (C) 2000 Elsevier Science Inc.
AB - The purpose of this study was to evaluate and compare two similar rodent memory tasks developed in our laboratory that employ stimulus discrimination and delayed response (light and tone stimuli and variable length delays) and to determine their sensitivity to the muscarinic-acetylcholine receptor (mAChR) antagonist, scopolamine hydrobromide (SCOP HBr), and its quaternary (methylbromide) analog (SCOP MBr). Male Wistar rats were trained in either an open chamber that employed retracting levers (RLM) during the delays, or a method that utilized closing doors (CDM) that separated the rats from the levers during delays to reduce positional (nonmnemonic) strategies. When the rats were well trained, dose-effect studies (μg/kg doses, s.c., 30 min before test sessions) of SCOP HBr or MBr were performed. Baseline performance was characterized by delay-dependent decreases in accuracy in both methods except when the tone was the stimulus in the RLM. SCOP HBr impaired performance in both tasks at the higher doses tested, although the effects were more consistent in the CDM task and accuracy associated with each stimulus was affected similarly. Surprisingly, SCOP MBr also impaired performance of each task, especially when the tone was the stimulus, while accuracy associated with the light was not affected in the CDM task. Overall, the results indicated that the CDM was a somewhat more reliable task, appearing to reduce positional strategies with less variability in response to the mAChR antagonists, although some stimulus-modality specific effects were noted. It also appears important to consider the peripheral effects of mAChR antagonists (and potential central effects of quaternary mAChR antagonists) when interpreting results from behavioral studies, especially those involving conditional discrimination and delayed response. (C) 2000 Elsevier Science Inc.
KW - Acetylcholine
KW - Antagonist
KW - Muscarinic
KW - Operant procedure
KW - Rat
KW - Scopolamine
KW - Stimulus discrimination
KW - Working memory
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U2 - 10.1016/S0031-9384(00)00233-X
DO - 10.1016/S0031-9384(00)00233-X
M3 - Article
C2 - 11006421
AN - SCOPUS:0033830732
SN - 0031-9384
VL - 70
SP - 233
EP - 241
JO - Physiology and Behavior
JF - Physiology and Behavior
IS - 3-4
ER -