Evidence that renal arginine transport is impaired in spontaneously hypertensive rats

N. W. Rajapakse, S. Kuruppu, I. Hanchapola, K. Venardos, D. L. Mattson, A. I. Smith, D. M. Kaye, R. G. Evans

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


Low renal nitric oxide (NO) bioavailability contributes to the development and maintenance of chronic hypertension. We investigated whether impaired L-arginine transport contributes to low renal NO bioavailability in hypertension. Responses of renal medullary perfusion and NO concentration to renal arterial infusions of the L-arginine transport inhibitor L-lysine (10 μmol·kg-1·min-1; 30 min) and subsequent superim-position of L-arginine (100 μmol·kg-1·min-1; 30 min), the NO synthase inhibitor N G-nitro-L-arginine (2.4 mg/kg; iv bolus), and the NO donor sodium nitroprusside (0.24 μg·kg-1·min-1) were examined in Sprague-Dawley rats (SD) and spontaneously hypertensive rats (SHR). Renal medullary perfusion and NO concentration were measured by laser-Doppler flowmetry and polarographically, respectively, 5.5 mm below the kidney surface. Renal medullary NO concentration was less in SHR (53 ± 3 nM) compared with SD rats (108 ±12 nM; P = 0.004). L-Lysine tended to reduce medullary perfusion (- 15 ± 7%; P = 0.07) and reduced medullary NO concentration (- 9 ± 3%; P = 0.03) while subsequent super imposition of L-arginine reversed these effects of L-lysine in SD rats. In SHR, L-lysine and subsequent superimposition of L-arginine did not significantly alter medullary perfusion or NO concentration. Collectively, these data suggest that renal L-arginine transport is impaired in SHR. Renal L-[3H]arginine transport was less in SHR compared with SD rats (P = 0.01). Accordingly, we conclude that impaired arginine transport contributes to low renal NO bioavailability observed in the SHR kidney.

Original languageEnglish (US)
Pages (from-to)F1554-F1562
JournalAmerican Journal of Physiology - Renal Physiology
Issue number12
StatePublished - 2012
Externally publishedYes


  • Hypertension
  • Kidney
  • L-arginine transport
  • Nitric oxide

ASJC Scopus subject areas

  • Physiology
  • Urology


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