Exosome secretome and mediated signaling in breast cancer patients with nontuberculous mycobacterial disease

Julie V. Philley; Anbarasu Kannan; David E. Griffith; Megan S. Devine; Jeana L. Benwill; Richard J. Wallace; Barbara A. Brown-Elliott; Foram Thakkar; Varsha Surendranath Taskar; James G. Fox; Ammar Alqaid; Hernaina Bains; Sudeep Gupta; Santanu Dasgupta

doi:10.18632/oncotarget.14964

Exosome secretome and mediated signaling in breast cancer patients with nontuberculous mycobacterial disease

Julie V. Philley, Anbarasu Kannan, David E. Griffith, Megan S. Devine, Jeana L. Benwill, Richard J. Wallace, Barbara A. Brown-Elliott, Foram Thakkar, Varsha Surendranath Taskar, James G. Fox, Ammar Alqaid, Hernaina Bains, Sudeep Gupta, Santanu Dasgupta

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Bronchiectasis Nontuberculous mycobacterium (NTM_nb) infection is an emerging health problem in breast cancer (BCa) patients. We measured sera exosome proteome in BCa-NTM_nb subjects and controls by Mass Spectroscopy. Extracellular matrix protein 1 (ECM1) was detected exclusively in the circulating exosomes of 82% of the BCa- NTM_nb cases. Co-culture of ECM1⁺ exosomes with normal human mammary epithelial cells induced epithelial to mesenchymal transition accompanied by increased Vimentin/CDH1 expression ratio and Glutamate production. Co-culture of the ECM1⁺ exosomes with normal human T cells modulated their cytokine production. The ECM1⁺ exosomes were markedly higher in sera obtained from BCa-NTM_nb subjects. Exclusive expression of APN, APOC4 and AZGP1 was evident in the circulating exosomes of these BCa-NTM_nb cases, which predicts disease prevalence independent of the body max index in concert with ECM1. Monitoring ECM1, APN, APOC4 and AZGP1 in the circulating exosomes could be beneficial for risk assessment, monitoring and surveillance of BCa-NTM_nb.

Original language	English (US)
Pages (from-to)	18070-18081
Number of pages	12
Journal	Oncotarget
Volume	8
Issue number	11
DOIs	https://doi.org/10.18632/oncotarget.14964
State	Published - 2017
Externally published	Yes

Keywords

Biomarker
Breast cancer
ECM1
Exosomes
Nontuberculous Mycobacterium

ASJC Scopus subject areas

Oncology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.18632/oncotarget.14964

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Philley, J. V., Kannan, A., Griffith, D. E., Devine, M. S., Benwill, J. L., Wallace, R. J., Brown-Elliott, B. A., Thakkar, F., Taskar, V. S., Fox, J. G., Alqaid, A., Bains, H., Gupta, S., & Dasgupta, S. (2017). Exosome secretome and mediated signaling in breast cancer patients with nontuberculous mycobacterial disease. Oncotarget, 8(11), 18070-18081. https://doi.org/10.18632/oncotarget.14964

Philley, JV, Kannan, A, Griffith, DE, Devine, MS, Benwill, JL, Wallace, RJ, Brown-Elliott, BA, Thakkar, F, Taskar, VS, Fox, JG, Alqaid, A, Bains, H, Gupta, S & Dasgupta, S 2017, 'Exosome secretome and mediated signaling in breast cancer patients with nontuberculous mycobacterial disease', Oncotarget, vol. 8, no. 11, pp. 18070-18081. https://doi.org/10.18632/oncotarget.14964

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abstract = "Bronchiectasis Nontuberculous mycobacterium (NTMnb) infection is an emerging health problem in breast cancer (BCa) patients. We measured sera exosome proteome in BCa-NTMnb subjects and controls by Mass Spectroscopy. Extracellular matrix protein 1 (ECM1) was detected exclusively in the circulating exosomes of 82% of the BCa- NTMnb cases. Co-culture of ECM1+ exosomes with normal human mammary epithelial cells induced epithelial to mesenchymal transition accompanied by increased Vimentin/CDH1 expression ratio and Glutamate production. Co-culture of the ECM1+ exosomes with normal human T cells modulated their cytokine production. The ECM1+ exosomes were markedly higher in sera obtained from BCa-NTMnb subjects. Exclusive expression of APN, APOC4 and AZGP1 was evident in the circulating exosomes of these BCa-NTMnb cases, which predicts disease prevalence independent of the body max index in concert with ECM1. Monitoring ECM1, APN, APOC4 and AZGP1 in the circulating exosomes could be beneficial for risk assessment, monitoring and surveillance of BCa-NTMnb.",

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AU - Benwill, Jeana L.

AU - Wallace, Richard J.

AU - Brown-Elliott, Barbara A.

AU - Thakkar, Foram

AU - Taskar, Varsha Surendranath

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AB - Bronchiectasis Nontuberculous mycobacterium (NTMnb) infection is an emerging health problem in breast cancer (BCa) patients. We measured sera exosome proteome in BCa-NTMnb subjects and controls by Mass Spectroscopy. Extracellular matrix protein 1 (ECM1) was detected exclusively in the circulating exosomes of 82% of the BCa- NTMnb cases. Co-culture of ECM1+ exosomes with normal human mammary epithelial cells induced epithelial to mesenchymal transition accompanied by increased Vimentin/CDH1 expression ratio and Glutamate production. Co-culture of the ECM1+ exosomes with normal human T cells modulated their cytokine production. The ECM1+ exosomes were markedly higher in sera obtained from BCa-NTMnb subjects. Exclusive expression of APN, APOC4 and AZGP1 was evident in the circulating exosomes of these BCa-NTMnb cases, which predicts disease prevalence independent of the body max index in concert with ECM1. Monitoring ECM1, APN, APOC4 and AZGP1 in the circulating exosomes could be beneficial for risk assessment, monitoring and surveillance of BCa-NTMnb.

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Exosome secretome and mediated signaling in breast cancer patients with nontuberculous mycobacterial disease

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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