Abstract
Objective: To test dysbindin-1 expression during spermatogenesis and dysbindin-1 effect on histology of acrosome in mice. Methods: Western blotting analysis was applied to detect the expression of dysbindin-1 in testis on day 7,14,21,28 and 35 postpartum and three-month old mice(n =3). The caudal spermatozoa was collected from sdy mice (no dysbindin-1 expression), HE staining was performed for sperm morphology, fluorescein isothiocyanate conjugate-pisum sativum agglutinin (FITC-PSA) staining and immunofluorescence staining with antibody against sperm protein 56 (sp56) for acrosome on sperm smear. Results: Dysbindin-1 A, not -1C, was expressed in postnatal testis development and spermatozoa. Mature sperm from sdy caudal epididymis had normal formation. Conclusion: Dysbindin-1 A, expressed in testis tissue and sperm during spermatogenesis, is not required for maintaining the morphology of sperm.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 548-552 |
| Number of pages | 5 |
| Journal | Acta Anatomica Sinica |
| Volume | 46 |
| Issue number | 4 |
| DOIs | |
| State | Published - Aug 2015 |
| Externally published | Yes |
Keywords
- Acrosome
- Dysbindin-1
- Mouse
- Sdy mouse
- Sperm
- Testis
- Western blotting
ASJC Scopus subject areas
- Anatomy
- Structural Biology
- Histology
- Embryology
Fingerprint
Dive into the research topics of 'Expression of dysbindin-1 during spermatogenesis'. Together they form a unique fingerprint.Cite this
- APA
- Standard
- Harvard
- Vancouver
- Author
- BIBTEX
- RIS