Abstract
Ultraviolet damage endonuclease (UVDE) is a 68.7 kDa DNA repair enzyme of Schizosaccharomyces pombe that recognizes cyclobutane pyrimidine dimers (CPDs) and 6-4 photoproducts (6-4 PPs). UVDE is thought to initiate the first step in an alternative excision repair pathway for removal of UV light- induced DNA damage. We have overexpressed Δ228-UVDE, an active truncated form of UVDE, and have purified this protein to apparent homogeneity. We have characterized purified Δ228-UVDE with respect to its physical properties, divalent cation requirements, and kinetic parameters on oligodeoxynucleotide substrates containing a single CPD. DNA strand cleavage analysis indicates that both full-length UVDE and Δ228-UVDE incise the CPD-containing strand immediately 5' to the lesion. These results provide further insight into the UVDE-mediated alternative excision repair pathway.
Original language | English (US) |
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Pages (from-to) | 11599-11604 |
Number of pages | 6 |
Journal | Biochemistry |
Volume | 37 |
Issue number | 33 |
DOIs | |
State | Published - Aug 18 1998 |
Externally published | Yes |
ASJC Scopus subject areas
- Biochemistry