TY - JOUR
T1 - Fluorescence of matrix isolated guanine and 7-methylguanine
AU - Polewski, Krzysztof
AU - Zinger, David
AU - Trunk, John
AU - Monteleone, Denise C.
AU - Sutherland, John C.
N1 - Funding Information:
Laboratory is supported by the Office of Energy Research, US Department of Energy.
Funding Information:
We thank Dr. Patrick Callis, Montana State University and Dr. Robert Woody, Colorado State University, for their insightful comments, Dr. Jack Preses, Chemistry Department, Brookhaven National Laboratory, for useful discussion concerning the time-resolved fluorescence measurements,a nd Ms. Ann Emrick for proofreading the manuscript. This research was supported by a grant from the National Institutes of Health (GM35662) and by the Office of Health and Environmental Research, US Department of Energy. The National Synchrotron Light Source at Brookhaven National
PY - 1994/8
Y1 - 1994/8
N2 - We have prepared argon and nitrogen matrices containing guanine and 7-methylguanine, and measured their absorption, fluorescence excitation and fluorescence emission spectra. The fluorescence excitation spectrum of guanine shows four well-resolved bands in the range from 170 to 290 nm; excitation at the wavelengths of each of these bands results in a fluorescence emission with maximum intensity near 350 nm and a single-exponential decay with a lifetime of about 10 ns. There are significant differences between the fluorescent excitation and emission spectra of guanine and of 7-methylguanine, suggesting that the fluorescence observed from the guanine sample does not arise from a minority tautomer.
AB - We have prepared argon and nitrogen matrices containing guanine and 7-methylguanine, and measured their absorption, fluorescence excitation and fluorescence emission spectra. The fluorescence excitation spectrum of guanine shows four well-resolved bands in the range from 170 to 290 nm; excitation at the wavelengths of each of these bands results in a fluorescence emission with maximum intensity near 350 nm and a single-exponential decay with a lifetime of about 10 ns. There are significant differences between the fluorescent excitation and emission spectra of guanine and of 7-methylguanine, suggesting that the fluorescence observed from the guanine sample does not arise from a minority tautomer.
KW - Absorption
KW - Electronic excited states
KW - Fluorescence
KW - Matrix isolation
KW - Phosphorescence
KW - Time-resolved fluorescence
UR - http://www.scopus.com/inward/record.url?scp=0028040690&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028040690&partnerID=8YFLogxK
U2 - 10.1016/1011-1344(94)07018-0
DO - 10.1016/1011-1344(94)07018-0
M3 - Article
C2 - 7965413
AN - SCOPUS:0028040690
SN - 1011-1344
VL - 24
SP - 169
EP - 177
JO - Journal of Photochemistry and Photobiology, B: Biology
JF - Journal of Photochemistry and Photobiology, B: Biology
IS - 3
ER -