Folic acid-decorated polyamidoamine dendrimer mediates selective uptake and high expression of genes in head and neck cancer cells

Leyuan Xu; Shannon Kittrell; W. Andrew Yeudall; Hu Yang

doi:10.2217/nnm-2016-0244

Folic acid-decorated polyamidoamine dendrimer mediates selective uptake and high expression of genes in head and neck cancer cells

Leyuan Xu, Shannon Kittrell, W. Andrew Yeudall, Hu Yang

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

Aim: Folic acid (FA)-decorated polyamidoamine dendrimer G4 (G4-FA) was synthesized and studied for targeted delivery of genes to head and neck cancer cells expressing high levels of folate receptors (FRs). Methods: Cellular uptake, targeting specificity, cytocompatibility and transfection efficiency were evaluated. Results: G4-FA competes with free FA for the same binding site. G4-FA facilitates the cellular uptake of DNA plasmids in a FR-dependent manner and selectively delivers plasmids to FR-high cells, leading to enhanced gene expression. Conclusion: G4-FA is a suitable vector to deliver genes selectively to head and neck cancer cells. The fundamental understandings of G4-FA as a vector and its encouraging transfection results for head and neck cancer cells provided support for its further testing in vivo.

Original language	English (US)
Pages (from-to)	2959-2973
Number of pages	15
Journal	Nanomedicine
Volume	11
Issue number	22
DOIs	https://doi.org/10.2217/nnm-2016-0244
State	Published - Nov 2016

Keywords

PAMAM dendrimer
folate receptor targeting
gene therapy
head and neck squamous cell carcinoma

ASJC Scopus subject areas

Bioengineering
Medicine (miscellaneous)
Biomedical Engineering
Materials Science(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.2217/nnm-2016-0244

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title = "Folic acid-decorated polyamidoamine dendrimer mediates selective uptake and high expression of genes in head and neck cancer cells",

abstract = "Aim: Folic acid (FA)-decorated polyamidoamine dendrimer G4 (G4-FA) was synthesized and studied for targeted delivery of genes to head and neck cancer cells expressing high levels of folate receptors (FRs). Methods: Cellular uptake, targeting specificity, cytocompatibility and transfection efficiency were evaluated. Results: G4-FA competes with free FA for the same binding site. G4-FA facilitates the cellular uptake of DNA plasmids in a FR-dependent manner and selectively delivers plasmids to FR-high cells, leading to enhanced gene expression. Conclusion: G4-FA is a suitable vector to deliver genes selectively to head and neck cancer cells. The fundamental understandings of G4-FA as a vector and its encouraging transfection results for head and neck cancer cells provided support for its further testing in vivo.",

keywords = "PAMAM dendrimer, folate receptor targeting, gene therapy, head and neck squamous cell carcinoma",

author = "Leyuan Xu and Shannon Kittrell and Yeudall, {W. Andrew} and Hu Yang",

note = "Funding Information: This work was supported, in part, by the National Science Foundation (CAREER award CBET0954957), and National Institutes of Health (R01EY024072 and R01DE024381). Confocal microscopy was performed at the VCU Department of Anatomy and Neurobiology Microscopy Facility, supported, in part, by funding from NIH-NINDS Center Core Grant P30NS047463 and, in part, by funding from NIH-NCI Cancer Center Support Grant P30CA016059. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed Publisher Copyright: {\textcopyright} 2016 Future Medicine Ltd.",

year = "2016",

month = nov,

doi = "10.2217/nnm-2016-0244",

language = "English (US)",

volume = "11",

pages = "2959--2973",

journal = "Nanomedicine",

issn = "1743-5889",

publisher = "Future Medicine Ltd.",

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T1 - Folic acid-decorated polyamidoamine dendrimer mediates selective uptake and high expression of genes in head and neck cancer cells

AU - Xu, Leyuan

AU - Kittrell, Shannon

AU - Yeudall, W. Andrew

AU - Yang, Hu

N1 - Funding Information: This work was supported, in part, by the National Science Foundation (CAREER award CBET0954957), and National Institutes of Health (R01EY024072 and R01DE024381). Confocal microscopy was performed at the VCU Department of Anatomy and Neurobiology Microscopy Facility, supported, in part, by funding from NIH-NINDS Center Core Grant P30NS047463 and, in part, by funding from NIH-NCI Cancer Center Support Grant P30CA016059. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed Publisher Copyright: © 2016 Future Medicine Ltd.

PY - 2016/11

Y1 - 2016/11

N2 - Aim: Folic acid (FA)-decorated polyamidoamine dendrimer G4 (G4-FA) was synthesized and studied for targeted delivery of genes to head and neck cancer cells expressing high levels of folate receptors (FRs). Methods: Cellular uptake, targeting specificity, cytocompatibility and transfection efficiency were evaluated. Results: G4-FA competes with free FA for the same binding site. G4-FA facilitates the cellular uptake of DNA plasmids in a FR-dependent manner and selectively delivers plasmids to FR-high cells, leading to enhanced gene expression. Conclusion: G4-FA is a suitable vector to deliver genes selectively to head and neck cancer cells. The fundamental understandings of G4-FA as a vector and its encouraging transfection results for head and neck cancer cells provided support for its further testing in vivo.

AB - Aim: Folic acid (FA)-decorated polyamidoamine dendrimer G4 (G4-FA) was synthesized and studied for targeted delivery of genes to head and neck cancer cells expressing high levels of folate receptors (FRs). Methods: Cellular uptake, targeting specificity, cytocompatibility and transfection efficiency were evaluated. Results: G4-FA competes with free FA for the same binding site. G4-FA facilitates the cellular uptake of DNA plasmids in a FR-dependent manner and selectively delivers plasmids to FR-high cells, leading to enhanced gene expression. Conclusion: G4-FA is a suitable vector to deliver genes selectively to head and neck cancer cells. The fundamental understandings of G4-FA as a vector and its encouraging transfection results for head and neck cancer cells provided support for its further testing in vivo.

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KW - gene therapy

KW - head and neck squamous cell carcinoma

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Folic acid-decorated polyamidoamine dendrimer mediates selective uptake and high expression of genes in head and neck cancer cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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