Fractionation of Pneumocystis carinii developmental stages by counterflow centrifugal elutriation and sequential filtrations

J. A. De Stefano; J. M. Foy; D. W. Sullivan; S. M. Dawes; M. T. Cushion; G. F. Babcock; R. G. Sleight; H. van Halbeek; P. D. Walzer

doi:10.1007/BF00932616

Fractionation of Pneumocystis carinii developmental stages by counterflow centrifugal elutriation and sequential filtrations

J. A. De Stefano, J. M. Foy, D. W. Sullivan, S. M. Dawes, M. T. Cushion, G. F. Babcock, R. G. Sleight, H. van Halbeek, P. D. Walzer

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Abstract

Immunomagnetic sorting, sequential filtrations, and counterflow centrifugal elutriation were compared for their ability to obtain enriched populations of Pneumocystis carinii developmental stages from infected rat-lung homogenates. Elutriation combined with sequential filtrations resulted in highly (>95%) enriched populations of P. carinii cysts and trophozoites with excellent viability. This approach offers advantages over previously described methods of obtaining enriched P. carinii cell populations and should have important applications to research on this organism.

Original language	English (US)
Pages (from-to)	1-9
Number of pages	9
Journal	Parasitology Research
Volume	80
Issue number	1
DOIs	https://doi.org/10.1007/BF00932616
State	Published - Jan 1994

ASJC Scopus subject areas

Parasitology
General Veterinary
Insect Science
Infectious Diseases

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/BF00932616

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title = "Fractionation of Pneumocystis carinii developmental stages by counterflow centrifugal elutriation and sequential filtrations",

abstract = "Immunomagnetic sorting, sequential filtrations, and counterflow centrifugal elutriation were compared for their ability to obtain enriched populations of Pneumocystis carinii developmental stages from infected rat-lung homogenates. Elutriation combined with sequential filtrations resulted in highly (>95%) enriched populations of P. carinii cysts and trophozoites with excellent viability. This approach offers advantages over previously described methods of obtaining enriched P. carinii cell populations and should have important applications to research on this organism.",

author = "{De Stefano}, {J. A.} and Foy, {J. M.} and Sullivan, {D. W.} and Dawes, {S. M.} and Cushion, {M. T.} and Babcock, {G. F.} and Sleight, {R. G.} and {van Halbeek}, H. and Walzer, {P. D.}",

year = "1994",

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AU - De Stefano, J. A.

AU - Foy, J. M.

AU - Sullivan, D. W.

AU - Dawes, S. M.

AU - Cushion, M. T.

AU - Babcock, G. F.

AU - Sleight, R. G.

AU - van Halbeek, H.

AU - Walzer, P. D.

PY - 1994/1

Y1 - 1994/1

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AB - Immunomagnetic sorting, sequential filtrations, and counterflow centrifugal elutriation were compared for their ability to obtain enriched populations of Pneumocystis carinii developmental stages from infected rat-lung homogenates. Elutriation combined with sequential filtrations resulted in highly (>95%) enriched populations of P. carinii cysts and trophozoites with excellent viability. This approach offers advantages over previously described methods of obtaining enriched P. carinii cell populations and should have important applications to research on this organism.

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Fractionation of Pneumocystis carinii developmental stages by counterflow centrifugal elutriation and sequential filtrations

Abstract

ASJC Scopus subject areas

UN SDGs

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