Full-length Mst1 exhibits growth promoting function in human hepatocellular carcinoma cells

Yuen Keng Ng; Wing Sze Lau; Vivian Wai Yan Lui; Alfred Sze Lok Cheng; Patrick Kwok Shing Ng; Stephen Kwok Wing Tsui; Yue Sun Cheung; Paul Bo San Lai

doi:10.1016/j.febslet.2013.01.018

Full-length Mst1 exhibits growth promoting function in human hepatocellular carcinoma cells

Yuen Keng Ng
, Wing Sze Lau
, Vivian Wai Yan Lui
, Alfred Sze Lok Cheng
, Patrick Kwok Shing Ng
, Stephen Kwok Wing Tsui
, Yue Sun Cheung
, Paul Bo San Lai

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The putative tumor suppressor Mst1, when cleaved to its 36 kDa cleaved form, amplifies apoptotic signals. We found that Mst1 was predominantly expressed in its full-length form in 76% (17/25 cases) of hepatocellular carcinoma (HCC) tumors. Mst1 cleavage was basically absent in HCC cells. Ectopic full-length Mst1 expression increased the growth of HCC cells by 55-80% within 3 days after transfection. Expression of exogenous NORE1B, a tumor suppressor commonly lost in HCC tumors (∼56% of our cohort), was sufficient to suppress the growth promotion of full-length Mst1. Hence, Mst1 exhibits a growth promoting activity in HCC cells upon NORE1B downregulation.

Original language	English (US)
Pages (from-to)	496-503
Number of pages	8
Journal	FEBS Letters
Volume	587
Issue number	5
DOIs	https://doi.org/10.1016/j.febslet.2013.01.018
State	Published - Mar 1 2013
Externally published	Yes

Keywords

Akt
Hep3B
Huh7
NORE1B
PLC/PRF/5
Staurosporine
Stk4

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2013.01.018

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title = "Full-length Mst1 exhibits growth promoting function in human hepatocellular carcinoma cells",

abstract = "The putative tumor suppressor Mst1, when cleaved to its 36 kDa cleaved form, amplifies apoptotic signals. We found that Mst1 was predominantly expressed in its full-length form in 76\% (17/25 cases) of hepatocellular carcinoma (HCC) tumors. Mst1 cleavage was basically absent in HCC cells. Ectopic full-length Mst1 expression increased the growth of HCC cells by 55-80\% within 3 days after transfection. Expression of exogenous NORE1B, a tumor suppressor commonly lost in HCC tumors (∼56\% of our cohort), was sufficient to suppress the growth promotion of full-length Mst1. Hence, Mst1 exhibits a growth promoting activity in HCC cells upon NORE1B downregulation.",

keywords = "Akt, Hep3B, Huh7, NORE1B, PLC/PRF/5, Staurosporine, Stk4",

author = "Ng, \{Yuen Keng\} and Lau, \{Wing Sze\} and Lui, \{Vivian Wai Yan\} and Cheng, \{Alfred Sze Lok\} and Ng, \{Patrick Kwok Shing\} and Tsui, \{Stephen Kwok Wing\} and Cheung, \{Yue Sun\} and Lai, \{Paul Bo San\}",

note = "Funding Information: This study is supported by the Direct Research Grant (2009.1.098) from the Chinese University of Hong Kong. The authors want to thank Dr. Yukiko Gotoh for providing us the myc-tagged full-length Mst1 mammalian expression plasmid. Thanks to Dr. Laura Stabile and Kathryn Supko for editing the manuscript. Also thanks to May Sui-Mui Li (Institute of Digestive Diseases) and the Surgical Research Laboratory of Department of Surgery, the Chinese University of Hong Kong for their technical supports. ",

year = "2013",

month = mar,

day = "1",

doi = "10.1016/j.febslet.2013.01.018",

language = "English (US)",

volume = "587",

pages = "496--503",

journal = "FEBS Letters",

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publisher = "John Wiley and Sons Inc.",

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T1 - Full-length Mst1 exhibits growth promoting function in human hepatocellular carcinoma cells

AU - Ng, Yuen Keng

AU - Lau, Wing Sze

AU - Lui, Vivian Wai Yan

AU - Cheng, Alfred Sze Lok

AU - Ng, Patrick Kwok Shing

AU - Tsui, Stephen Kwok Wing

AU - Cheung, Yue Sun

AU - Lai, Paul Bo San

N1 - Funding Information: This study is supported by the Direct Research Grant (2009.1.098) from the Chinese University of Hong Kong. The authors want to thank Dr. Yukiko Gotoh for providing us the myc-tagged full-length Mst1 mammalian expression plasmid. Thanks to Dr. Laura Stabile and Kathryn Supko for editing the manuscript. Also thanks to May Sui-Mui Li (Institute of Digestive Diseases) and the Surgical Research Laboratory of Department of Surgery, the Chinese University of Hong Kong for their technical supports.

PY - 2013/3/1

Y1 - 2013/3/1

N2 - The putative tumor suppressor Mst1, when cleaved to its 36 kDa cleaved form, amplifies apoptotic signals. We found that Mst1 was predominantly expressed in its full-length form in 76% (17/25 cases) of hepatocellular carcinoma (HCC) tumors. Mst1 cleavage was basically absent in HCC cells. Ectopic full-length Mst1 expression increased the growth of HCC cells by 55-80% within 3 days after transfection. Expression of exogenous NORE1B, a tumor suppressor commonly lost in HCC tumors (∼56% of our cohort), was sufficient to suppress the growth promotion of full-length Mst1. Hence, Mst1 exhibits a growth promoting activity in HCC cells upon NORE1B downregulation.

AB - The putative tumor suppressor Mst1, when cleaved to its 36 kDa cleaved form, amplifies apoptotic signals. We found that Mst1 was predominantly expressed in its full-length form in 76% (17/25 cases) of hepatocellular carcinoma (HCC) tumors. Mst1 cleavage was basically absent in HCC cells. Ectopic full-length Mst1 expression increased the growth of HCC cells by 55-80% within 3 days after transfection. Expression of exogenous NORE1B, a tumor suppressor commonly lost in HCC tumors (∼56% of our cohort), was sufficient to suppress the growth promotion of full-length Mst1. Hence, Mst1 exhibits a growth promoting activity in HCC cells upon NORE1B downregulation.

KW - Akt

KW - Hep3B

KW - Huh7

KW - NORE1B

KW - PLC/PRF/5

KW - Staurosporine

KW - Stk4

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UR - https://www.scopus.com/pages/publications/84874193499#tab=citedBy

U2 - 10.1016/j.febslet.2013.01.018

DO - 10.1016/j.febslet.2013.01.018

M3 - Article

C2 - 23347832

AN - SCOPUS:84874193499

SN - 0014-5793

VL - 587

SP - 496

EP - 503

JO - FEBS Letters

JF - FEBS Letters

IS - 5

ER -

Full-length Mst1 exhibits growth promoting function in human hepatocellular carcinoma cells

Abstract

Keywords

ASJC Scopus subject areas

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