TY - JOUR
T1 - Functional human corneal equivalents constructed from cell lines
AU - Griffith, May
AU - Osborne, Rosemarie
AU - Hunger, Rejean
AU - Xiong, Xiaojuan
AU - Doillon, Charles J.
AU - Laycock, Noelani L.C.
AU - Hakim, Malik
AU - Song, Ying
AU - Watsky, Mitchell A.
PY - 1999/12/10
Y1 - 1999/12/10
N2 - Human corneal equivalents comprising the three main layers of the cornea (epithelium, stroma, and endothelium) were constructed. Each cellular layer was fabricated from immortalized human corneal cells that were screened for use on the basis of morphological biochemical, and electrophysiological similarity to their natural counterparts. The resulting corneal equivalents mimicked hum an corneas in key physical and physiological functions including morphology, biochemical marker expression, transparency, ion and fluid transport, and gene expression. Morphological and functional equivalents to human corneas that can be produced in vitro have immediate applications in toxicity and drug efficacy testing, and form the basis for future development of implantable tissues.
AB - Human corneal equivalents comprising the three main layers of the cornea (epithelium, stroma, and endothelium) were constructed. Each cellular layer was fabricated from immortalized human corneal cells that were screened for use on the basis of morphological biochemical, and electrophysiological similarity to their natural counterparts. The resulting corneal equivalents mimicked hum an corneas in key physical and physiological functions including morphology, biochemical marker expression, transparency, ion and fluid transport, and gene expression. Morphological and functional equivalents to human corneas that can be produced in vitro have immediate applications in toxicity and drug efficacy testing, and form the basis for future development of implantable tissues.
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U2 - 10.1126/science.286.5447.2169
DO - 10.1126/science.286.5447.2169
M3 - Article
C2 - 10591651
AN - SCOPUS:0032797273
SN - 0036-8075
VL - 286
SP - 2169
EP - 2172
JO - Science
JF - Science
IS - 5447
ER -