TY - JOUR
T1 - Functional parathyroid hormone receptors are present in an umbilical vein endothelial cell line
AU - Isales, Carlos M
AU - Sumpio, Bauer
AU - Bollag, Roni Jacob
AU - Zhong, Qing
AU - Ding, Kehong
AU - Du, Wei
AU - Rodriguez-Commes, Jose
AU - Lopez, Raquel
AU - Rosales, Oscar R.
AU - Gasalla-Herraiz, Jose
AU - McCarthy, Richard
AU - Barrett, Paula Q.
PY - 2000
Y1 - 2000
N2 - Acute parathyroid hormone exposure induces vascular smooth muscle relaxation. In contrast, continuous infusion of parathyroid hormone leads to vasoconstriction and an elevation of blood pressure. Despite the known effects of parathyroid hormone on vascular smooth muscle, possible direct effects on the vascular endothelium have not previously been investigated. Using a human umbilical vein endothelial cell line, we found that parathyroid hormone increased both intracellular calcium and cellular cAMP content in these endothelial cells. Furthermore, exposure of these cells to increasing concentrations of parathyroid hormone stimulated both [3H]thymidine incorporation and endothelin-1 secretion. Parathyroid hormone/parathyroid hormone-related peptide receptor mRNA could be detected at low levels in these cells. In summary, these data demonstrate that endothelium-derived cells contain functional parathyroid hormone receptors. The potential physiological role of these receptors remains to be determined.
AB - Acute parathyroid hormone exposure induces vascular smooth muscle relaxation. In contrast, continuous infusion of parathyroid hormone leads to vasoconstriction and an elevation of blood pressure. Despite the known effects of parathyroid hormone on vascular smooth muscle, possible direct effects on the vascular endothelium have not previously been investigated. Using a human umbilical vein endothelial cell line, we found that parathyroid hormone increased both intracellular calcium and cellular cAMP content in these endothelial cells. Furthermore, exposure of these cells to increasing concentrations of parathyroid hormone stimulated both [3H]thymidine incorporation and endothelin-1 secretion. Parathyroid hormone/parathyroid hormone-related peptide receptor mRNA could be detected at low levels in these cells. In summary, these data demonstrate that endothelium-derived cells contain functional parathyroid hormone receptors. The potential physiological role of these receptors remains to be determined.
KW - Adenosine 3',5'-cyclic monophosphate
KW - Endothelial cells
KW - Intracellular calcium
KW - Parathyroid hormone
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U2 - 10.1152/ajpendo.2000.279.3.e654
DO - 10.1152/ajpendo.2000.279.3.e654
M3 - Article
C2 - 10950835
AN - SCOPUS:0033826344
SN - 0193-1849
VL - 279
SP - E654-E662
JO - American Journal of Physiology - Endocrinology and Metabolism
JF - American Journal of Physiology - Endocrinology and Metabolism
IS - 3 42-3
ER -
