Fungicidal lipid-transfer peptide from Daucus carota sativa seeds

A. Yili; H. A. Aisa; X. Imamu; R. H. Zhen; Q. Zhang; V. V. Maksimov; O. N. Veshkurova; Sh I. Salikhov

doi:10.1007/s10600-007-0158-2

Fungicidal lipid-transfer peptide from Daucus carota sativa seeds

A. Yili, H. A. Aisa, X. Imamu, R. H. Zhen, Q. Zhang, V. V. Maksimov, O. N. Veshkurova, Sh I. Salikhov

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

A non-specific lipid-transfer peptide (nsLTP) with fungicidal activity was isolated from Daucus carota sativa carrot seeds. Peptides were purified by a method including aqueous extraction, anion-exchange chromatography over CM-TSK-650M, and HPLC over a column of 250/8/4 Protein@Peptide C₁₈ using an acetonitrile gradient. The molecular weight of the peptide was determined as 9624 Da by mass spectrometry. The peptide was found to have fungicidal activity against the pathogenic fungus Verticillium dahliae. The partial N-terminal sequence, which was highly homologous to the N-terminal sequences of lipid-transfer peptides from seeds of rice, tobacco, and maize, was determined using Edman automated sequencing.

Original language	English (US)
Pages (from-to)	450-453
Number of pages	4
Journal	Chemistry of Natural Compounds
Volume	43
Issue number	4
DOIs	https://doi.org/10.1007/s10600-007-0158-2
State	Published - Jul 2007
Externally published	Yes

Keywords

Carrot seeds
Fungicidal lipid-transfer peptides
N-terminal amino-acid sequence

ASJC Scopus subject areas

Plant Science

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10.1007/s10600-007-0158-2

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@article{48e1cfc7ce9b42478da3b8e77ed34219,

title = "Fungicidal lipid-transfer peptide from Daucus carota sativa seeds",

abstract = "A non-specific lipid-transfer peptide (nsLTP) with fungicidal activity was isolated from Daucus carota sativa carrot seeds. Peptides were purified by a method including aqueous extraction, anion-exchange chromatography over CM-TSK-650M, and HPLC over a column of 250/8/4 Protein@Peptide C18 using an acetonitrile gradient. The molecular weight of the peptide was determined as 9624 Da by mass spectrometry. The peptide was found to have fungicidal activity against the pathogenic fungus Verticillium dahliae. The partial N-terminal sequence, which was highly homologous to the N-terminal sequences of lipid-transfer peptides from seeds of rice, tobacco, and maize, was determined using Edman automated sequencing.",

keywords = "Carrot seeds, Fungicidal lipid-transfer peptides, N-terminal amino-acid sequence",

author = "A. Yili and Aisa, {H. A.} and X. Imamu and Zhen, {R. H.} and Q. Zhang and Maksimov, {V. V.} and Veshkurova, {O. N.} and Salikhov, {Sh I.}",

note = "Funding Information: The work was performed with financial support from the PRC AS, the Laboratory of Natural Product Chemistry of Xinjiang Technical Institute of Physics and Chemistry, and the Coordination Committee for Development of Science and Technology of the Cabinet of Ministers of the Republic of Uzbekistan, grant FA-F3-T140.",

year = "2007",

month = jul,

doi = "10.1007/s10600-007-0158-2",

language = "English (US)",

volume = "43",

pages = "450--453",

journal = "Chemistry of Natural Compounds",

issn = "0009-3130",

publisher = "Springer New York",

number = "4",

}

TY - JOUR

T1 - Fungicidal lipid-transfer peptide from Daucus carota sativa seeds

AU - Yili, A.

AU - Aisa, H. A.

AU - Imamu, X.

AU - Zhen, R. H.

AU - Zhang, Q.

AU - Maksimov, V. V.

AU - Veshkurova, O. N.

AU - Salikhov, Sh I.

N1 - Funding Information: The work was performed with financial support from the PRC AS, the Laboratory of Natural Product Chemistry of Xinjiang Technical Institute of Physics and Chemistry, and the Coordination Committee for Development of Science and Technology of the Cabinet of Ministers of the Republic of Uzbekistan, grant FA-F3-T140.

PY - 2007/7

Y1 - 2007/7

N2 - A non-specific lipid-transfer peptide (nsLTP) with fungicidal activity was isolated from Daucus carota sativa carrot seeds. Peptides were purified by a method including aqueous extraction, anion-exchange chromatography over CM-TSK-650M, and HPLC over a column of 250/8/4 Protein@Peptide C18 using an acetonitrile gradient. The molecular weight of the peptide was determined as 9624 Da by mass spectrometry. The peptide was found to have fungicidal activity against the pathogenic fungus Verticillium dahliae. The partial N-terminal sequence, which was highly homologous to the N-terminal sequences of lipid-transfer peptides from seeds of rice, tobacco, and maize, was determined using Edman automated sequencing.

AB - A non-specific lipid-transfer peptide (nsLTP) with fungicidal activity was isolated from Daucus carota sativa carrot seeds. Peptides were purified by a method including aqueous extraction, anion-exchange chromatography over CM-TSK-650M, and HPLC over a column of 250/8/4 Protein@Peptide C18 using an acetonitrile gradient. The molecular weight of the peptide was determined as 9624 Da by mass spectrometry. The peptide was found to have fungicidal activity against the pathogenic fungus Verticillium dahliae. The partial N-terminal sequence, which was highly homologous to the N-terminal sequences of lipid-transfer peptides from seeds of rice, tobacco, and maize, was determined using Edman automated sequencing.

KW - Carrot seeds

KW - Fungicidal lipid-transfer peptides

KW - N-terminal amino-acid sequence

UR - http://www.scopus.com/inward/record.url?scp=36049020668&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=36049020668&partnerID=8YFLogxK

U2 - 10.1007/s10600-007-0158-2

DO - 10.1007/s10600-007-0158-2

M3 - Article

AN - SCOPUS:36049020668

SN - 0009-3130

VL - 43

SP - 450

EP - 453

JO - Chemistry of Natural Compounds

JF - Chemistry of Natural Compounds

IS - 4

ER -

Fungicidal lipid-transfer peptide from Daucus carota sativa seeds

Abstract

Keywords

ASJC Scopus subject areas

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