Further characterization of tetracycline's quantitative binding to dentin

Alfred E. Ciarlone, Richard D. Johnson, David H. Pashley

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Unerupted human third molar crown segments were used to measure the change in the concentration of 3H-tetracycline that occurs when it moves across dentin from pulpal to occlusal surfaces. When 1.25, 2.5, and 5×10-5M3H-tetracycline were filtered across dentin, binding increased by 21, 51, and 69%, respectively. When the same concentrations of 3H-tetracycline were preloaded onto crown segments and eluted with 1.1×10-3M nonradioactive tetracycline, the percentage of elution of 3H-tetracycline was not significantly different over time. When preloaded 3H-tetracycline was displaced by diffusion with water or 1.1×10-3M nonradioactive tetracycline, there were no significant differences. There was a significant difference between the rate of displacement of 1.25×10-5M3H-tetracycline and the other two preloaded concentrations when 5.8×10-4M3H-tetracycline/tetracycline was used as the eluant. These data suggest that the binding of 3H-tetracycline to dentin was concentration dependent and that 3H-tetracycline binds nonspecifically and reversibly.

Original languageEnglish (US)
Pages (from-to)335-338
Number of pages4
JournalJournal of endodontics
Issue number8
StatePublished - Aug 1989

ASJC Scopus subject areas

  • Dentistry(all)


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