G-protein-coupled receptor interaction with small GTPases

Chunmin Dong, Guangyu Wu

Research output: Chapter in Book/Report/Conference proceedingChapter

9 Scopus citations


In addition to heterotrimeric G-proteins, Ras-like small GTPases are also involved in regulating physiological functions of the G-protein-coupled receptor (GPCR) superfamily. In particular, Rab and ARF GTPases function either as "traffic cops" to coordinate receptor targeting to specific locations or as "signal transducers" to directly mediate receptor signal propagation. As revealed in protein-protein interaction assays, GPCRs may use specific motifs to physically interact with small GTPases, providing important insights into the underlying molecular mechanisms. In this chapter, we describe coimmunoprecipitation and GST fusion protein pull-down approaches to study the GPCR-small GTPase interaction, by focusing on the interaction of α2B- and β2-adrenegic receptors with the small GTPases Rab8 and ARF1.

Original languageEnglish (US)
Title of host publicationG Protein Coupled ReceptorsModeling, Activation, Interactions and Virtual Screening
PublisherAcademic Press Inc.
Number of pages12
ISBN (Print)9780124078659
StatePublished - 2013

Publication series

NameMethods in Enzymology
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988


  • ARF1
  • G-protein-coupled receptor
  • Protein-protein interaction
  • Rab8
  • Signaling
  • Small GTPases
  • Trafficking

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology


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