TY - JOUR
T1 - GANGLIOSIDES OF HUMAN MYELIN
T2 - SIALOSYLGALACTOSYLCERAMIDE (G7) AS A MAJOR COMPONENT
AU - Ledeen, R. W.
AU - Yu, R. K.
AU - Eng, L. F.
PY - 1973/10
Y1 - 1973/10
N2 - Abstract— Gangliosides were isolated from purified human myelin in a yield of 62 μg of lipid‐bound sialic acid per 100 mg of dry myelin. Sialosylgalactosyl ceramide (G7) was found to be a major component of the ganglioside fraction, amounting to 15 per cent of the total sialic acid. It accounted for 10 per cent of lipid‐bound sialic acid in adult human white matter, making it the third most abundant ganglioside on a molar basis. These results were obtained with an improved method for isolating total gangliosides in high yield, by employing DEAE‐Sephadex column chromatography. Myelin from other mammalian species had considerably less G7, and there were also indications of maturational changes. Both 2‐hydroxy and unsubstituted fatty acids were components of the ceramide unit, in a ratio of 3:2, respectively. The overall fatty acid pattern was very similar to that for myelin cerebroside and sulphatide. Long‐chain bases included only C18 species, with sphingosine predominating (>90 per cent). These observations suggest a metabolic relationship between G7 and either cerebroside or sulphatide.
AB - Abstract— Gangliosides were isolated from purified human myelin in a yield of 62 μg of lipid‐bound sialic acid per 100 mg of dry myelin. Sialosylgalactosyl ceramide (G7) was found to be a major component of the ganglioside fraction, amounting to 15 per cent of the total sialic acid. It accounted for 10 per cent of lipid‐bound sialic acid in adult human white matter, making it the third most abundant ganglioside on a molar basis. These results were obtained with an improved method for isolating total gangliosides in high yield, by employing DEAE‐Sephadex column chromatography. Myelin from other mammalian species had considerably less G7, and there were also indications of maturational changes. Both 2‐hydroxy and unsubstituted fatty acids were components of the ceramide unit, in a ratio of 3:2, respectively. The overall fatty acid pattern was very similar to that for myelin cerebroside and sulphatide. Long‐chain bases included only C18 species, with sphingosine predominating (>90 per cent). These observations suggest a metabolic relationship between G7 and either cerebroside or sulphatide.
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U2 - 10.1111/j.1471-4159.1973.tb07527.x
DO - 10.1111/j.1471-4159.1973.tb07527.x
M3 - Article
C2 - 4754859
AN - SCOPUS:0015822234
SN - 0022-3042
VL - 21
SP - 829
EP - 839
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
IS - 4
ER -