Gangliosides of normal and neoplastic human melanocytes

John M. Carubia; Robert K. Yu; Larry J. Macala; John M. Kirkwood; Janos M. Varga

doi:10.1016/0006-291X(84)91282-8

Gangliosides of normal and neoplastic human melanocytes

John M. Carubia, Robert K. Yu, Larry J. Macala, John M. Kirkwood, Janos M. Varga

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Abstract

The major ganglioside component isolated from diploid human melanocytes is sialosyllactosylceramide (G_M3 86-91% of total sialic acid). The corresponding disialo derivative (G_D3) is found as a minor component (2-6% of total sialic acid) in the membranes of these cells. In human melanoma cells, grown in tissue culture, G_D3 is the predominant ganglioside component (48-63% of total sialic acid). Withdrawal of TPA from the culture medium of normal melanocytes or addition of TPA to the medium of melanoma cells had no significant effect on G_M3 G_D3 ratios. We conclude that the difference between the composition of gangliosides is related to the normal vs transformed phenotypes of melanocytes.

Original language	English (US)
Pages (from-to)	500-504
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	120
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(84)91282-8
State	Published - Apr 30 1984
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(84)91282-8

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title = "Gangliosides of normal and neoplastic human melanocytes",

abstract = "The major ganglioside component isolated from diploid human melanocytes is sialosyllactosylceramide (GM3 86-91% of total sialic acid). The corresponding disialo derivative (GD3) is found as a minor component (2-6% of total sialic acid) in the membranes of these cells. In human melanoma cells, grown in tissue culture, GD3 is the predominant ganglioside component (48-63% of total sialic acid). Withdrawal of TPA from the culture medium of normal melanocytes or addition of TPA to the medium of melanoma cells had no significant effect on GM3 GD3 ratios. We conclude that the difference between the composition of gangliosides is related to the normal vs transformed phenotypes of melanocytes.",

author = "Carubia, {John M.} and Yu, {Robert K.} and Macala, {Larry J.} and Kirkwood, {John M.} and Varga, {Janos M.}",

note = "Funding Information: These studies were supported by grant No. CA 26081 from the National Cancer Institute and from a grant by the National Foundation for Cancer Research. We thank Mrs. G. Herbert and .Ms. Tracy Bobak for their help with tissue cultures and Mrs. Elena DiMassa for typing tne manuscript.",

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doi = "10.1016/0006-291X(84)91282-8",

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pages = "500--504",

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TY - JOUR

T1 - Gangliosides of normal and neoplastic human melanocytes

AU - Carubia, John M.

AU - Yu, Robert K.

AU - Macala, Larry J.

AU - Kirkwood, John M.

AU - Varga, Janos M.

N1 - Funding Information: These studies were supported by grant No. CA 26081 from the National Cancer Institute and from a grant by the National Foundation for Cancer Research. We thank Mrs. G. Herbert and .Ms. Tracy Bobak for their help with tissue cultures and Mrs. Elena DiMassa for typing tne manuscript.

PY - 1984/4/30

Y1 - 1984/4/30

N2 - The major ganglioside component isolated from diploid human melanocytes is sialosyllactosylceramide (GM3 86-91% of total sialic acid). The corresponding disialo derivative (GD3) is found as a minor component (2-6% of total sialic acid) in the membranes of these cells. In human melanoma cells, grown in tissue culture, GD3 is the predominant ganglioside component (48-63% of total sialic acid). Withdrawal of TPA from the culture medium of normal melanocytes or addition of TPA to the medium of melanoma cells had no significant effect on GM3 GD3 ratios. We conclude that the difference between the composition of gangliosides is related to the normal vs transformed phenotypes of melanocytes.

AB - The major ganglioside component isolated from diploid human melanocytes is sialosyllactosylceramide (GM3 86-91% of total sialic acid). The corresponding disialo derivative (GD3) is found as a minor component (2-6% of total sialic acid) in the membranes of these cells. In human melanoma cells, grown in tissue culture, GD3 is the predominant ganglioside component (48-63% of total sialic acid). Withdrawal of TPA from the culture medium of normal melanocytes or addition of TPA to the medium of melanoma cells had no significant effect on GM3 GD3 ratios. We conclude that the difference between the composition of gangliosides is related to the normal vs transformed phenotypes of melanocytes.

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DO - 10.1016/0006-291X(84)91282-8

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SN - 0006-291X

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EP - 504

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Gangliosides of normal and neoplastic human melanocytes

Abstract

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