GCSF receptor regulates antigen uptake and expression of cytokines and costimulatory molecules in dendritic cells

Degui Geng; Sunil K. Joshi; Robert Podolsky; Jin Xiong She

doi:10.1016/j.molimm.2006.02.009

GCSF receptor regulates antigen uptake and expression of cytokines and costimulatory molecules in dendritic cells

Degui Geng
, Sunil K. Joshi
, Robert Podolsky
, Jin Xiong She

Obstetrics and Gynecology

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

RNA interference (RNAi), a process that specifically silences target gene expression, is a powerful technique to modulate cellular functions. In this study, we identified two small interference RNA (siRNA) sequences that can specifically and efficiently silence the expression of the granulocyte colony-stimulating factor receptor (GCSF-R) gene and achieved stable knockdown of GCSF-R using pFIV lentivirus containing the GCSF-R siRNA. GCSF-R knockdown significantly reduces the expression of IL-lα, IL-lβ, IL-6, IL-10, H-2K^b, I-A^b, CD80 and CD86, and increases PDL1 and PDL2 expression, while IL-12p35, TGFβ, TNFα and CD40 expression is unaltered. Furthermore, GCSF-R knockdown significantly changes the endocytosis and micro-pinocytosis abilities as well as surface expression of antigens of DC2.4 cells.

Original language	English (US)
Pages (from-to)	521-529
Number of pages	9
Journal	Molecular Immunology
Volume	44
Issue number	4
DOIs	https://doi.org/10.1016/j.molimm.2006.02.009
State	Published - Jan 2007

Keywords

Antigen presentation
Antigen uptake
Dendritic cells
GCSF
GCSF-R
siRNA

ASJC Scopus subject areas

Immunology
Molecular Biology

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10.1016/j.molimm.2006.02.009

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title = "GCSF receptor regulates antigen uptake and expression of cytokines and costimulatory molecules in dendritic cells",

abstract = "RNA interference (RNAi), a process that specifically silences target gene expression, is a powerful technique to modulate cellular functions. In this study, we identified two small interference RNA (siRNA) sequences that can specifically and efficiently silence the expression of the granulocyte colony-stimulating factor receptor (GCSF-R) gene and achieved stable knockdown of GCSF-R using pFIV lentivirus containing the GCSF-R siRNA. GCSF-R knockdown significantly reduces the expression of IL-lα, IL-lβ, IL-6, IL-10, H-2Kb, I-Ab, CD80 and CD86, and increases PDL1 and PDL2 expression, while IL-12p35, TGFβ, TNFα and CD40 expression is unaltered. Furthermore, GCSF-R knockdown significantly changes the endocytosis and micro-pinocytosis abilities as well as surface expression of antigens of DC2.4 cells.",

keywords = "Antigen presentation, Antigen uptake, Dendritic cells, GCSF, GCSF-R, siRNA",

author = "Degui Geng and Joshi, \{Sunil K.\} and Robert Podolsky and She, \{Jin Xiong\}",

note = "Funding Information: This work was partly supported by grants from the National Institutes of Health (2RO1HD37800, 2P01 AI-42288, 5U24DK58778, 1R21HD050196, 121DK069878, and JDRF 1-2004-661) to JXS. ",

year = "2007",

month = jan,

doi = "10.1016/j.molimm.2006.02.009",

language = "English (US)",

volume = "44",

pages = "521--529",

journal = "Molecular Immunology",

issn = "0161-5890",

publisher = "Elsevier Ltd",

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T1 - GCSF receptor regulates antigen uptake and expression of cytokines and costimulatory molecules in dendritic cells

AU - Geng, Degui

AU - Joshi, Sunil K.

AU - Podolsky, Robert

AU - She, Jin Xiong

N1 - Funding Information: This work was partly supported by grants from the National Institutes of Health (2RO1HD37800, 2P01 AI-42288, 5U24DK58778, 1R21HD050196, 121DK069878, and JDRF 1-2004-661) to JXS.

PY - 2007/1

Y1 - 2007/1

N2 - RNA interference (RNAi), a process that specifically silences target gene expression, is a powerful technique to modulate cellular functions. In this study, we identified two small interference RNA (siRNA) sequences that can specifically and efficiently silence the expression of the granulocyte colony-stimulating factor receptor (GCSF-R) gene and achieved stable knockdown of GCSF-R using pFIV lentivirus containing the GCSF-R siRNA. GCSF-R knockdown significantly reduces the expression of IL-lα, IL-lβ, IL-6, IL-10, H-2Kb, I-Ab, CD80 and CD86, and increases PDL1 and PDL2 expression, while IL-12p35, TGFβ, TNFα and CD40 expression is unaltered. Furthermore, GCSF-R knockdown significantly changes the endocytosis and micro-pinocytosis abilities as well as surface expression of antigens of DC2.4 cells.

AB - RNA interference (RNAi), a process that specifically silences target gene expression, is a powerful technique to modulate cellular functions. In this study, we identified two small interference RNA (siRNA) sequences that can specifically and efficiently silence the expression of the granulocyte colony-stimulating factor receptor (GCSF-R) gene and achieved stable knockdown of GCSF-R using pFIV lentivirus containing the GCSF-R siRNA. GCSF-R knockdown significantly reduces the expression of IL-lα, IL-lβ, IL-6, IL-10, H-2Kb, I-Ab, CD80 and CD86, and increases PDL1 and PDL2 expression, while IL-12p35, TGFβ, TNFα and CD40 expression is unaltered. Furthermore, GCSF-R knockdown significantly changes the endocytosis and micro-pinocytosis abilities as well as surface expression of antigens of DC2.4 cells.

KW - Antigen presentation

KW - Antigen uptake

KW - Dendritic cells

KW - GCSF

KW - GCSF-R

KW - siRNA

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U2 - 10.1016/j.molimm.2006.02.009

DO - 10.1016/j.molimm.2006.02.009

M3 - Article

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AN - SCOPUS:33748600398

SN - 0161-5890

VL - 44

SP - 521

EP - 529

JO - Molecular Immunology

JF - Molecular Immunology

IS - 4

ER -

GCSF receptor regulates antigen uptake and expression of cytokines and costimulatory molecules in dendritic cells

Abstract

Keywords

ASJC Scopus subject areas

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