Gene-targeted mice reveal importance of L-selectin-dependent rolling for neutrophil adhesion

Unsu Jung, Carroll L. Ramos, Daniel C. Bullard, Klaus Ley

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


It has not been determined whether L-selectin-mediated rolling can promote leukocyte adhesion in vivo independent of P- and E-selectin. We used intravital microscopy of E- and P-selectin double-mutant mice (E-/P-) stimulated with tumor necrosis factor-α for 6-8 h to investigate the importance of L-selectin-dependent rolling in cremaster muscle venules. Rolling leukocyte flux in E-/P- mice was 9 ± 2 cells/min compared with 77 ± 17 cells/min in wild-type (WT) mice. Pretreatment with the L-selectin monoclonal antibody MEL-14 significantly reduced rolling in both E-/P(by 89%) and WT mice (by 79%). L-selectin-dependent rolling in E-/P- mice resulted in leukocyte adhesion comparable to that seen in WT mice. MEL-14 pretreatment of E-/P- mice reduced leukocyte adhesion by 50%. The majority (~80%) of intravascular leukocytes in both WT and E-/P- mice were neutrophils. We conclude that L-selectin can mediate rolling that results in sufficient leukocyte recruitment to account for the robust inflammatory response seen in E-/P- mice at later times.

Original languageEnglish (US)
Pages (from-to)H1785-H1791
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Issue number5 43-5
StatePublished - May 1998
Externally publishedYes


  • Inflammation
  • Intravital microscopy
  • Knockout mice
  • Leukocyte adhesion

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)


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