Generation of Sigmar1 conditional knockout mouse using CRISPR-Cas9 gene targeting

Liang Huang, Haiyan Xiao, Xiaoling Xie, Fang Hu, Fulei Tang, Sylvia B. Smith, Lin Gan

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


The Sigma 1 receptor (SIGMAR1) is a transmembrane protein located in the mitochondria-associated endoplasmic reticulum membrane, and plays an important role in cell survival as a pluripotent modulator of a variety of signaling pathways related to neurodegeneration. Though SIGMAR1 is a potential target for neurodegenerative diseases, the specific role of SIGMAR1 in different tissue and cell types remains unclear. Here we reported the generation of Sigmar1 conditional knockout (Sigmar1loxP) mice using CRISPR-Cas9 method to insert loxP sites into the 5′- and 3′-untranslated regions of Sigmar1. We showed that the insertion of loxP sequences did not affect the expression of Sigmar1 and that Sigmar1loxP/loxP mice exhibited no detectable visual defects compared with wild-type mice at the early adult stage. By crossing Sigmar1loxP mice with retina-specific Six3-Cre and ubiquitous CMV-Cre mice, we confirmed the deletion of Sigmar1 coding regions of exons 1–4, and the retina-specific and global loss of SIGMAR1 expression, respectively. Thus, Sigmar1loxP mice provide a valuable tool for unraveling the tissue and cell-type-specific role of Sigmar1.

Original languageEnglish (US)
Article numbere23487
Issue number6-7
StatePublished - Jul 2022


  • CRISPR-Cas9
  • retina
  • sconditional knockout
  • sigma receptor

ASJC Scopus subject areas

  • Genetics
  • Endocrinology
  • Cell Biology


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