TY - JOUR
T1 - Genistein decreases food intake, body weight, and fat pad weight and causes adipose tissue apoptosis in ovariectomized female mice
AU - Kim, Hye Kyeong
AU - Nelson-Dooley, Cassandra
AU - Della-Fera, Mary Anne
AU - Yang, Jeong Yeh
AU - Zhang, Wei
AU - Duan, Jiuhua
AU - Hartzell, Diane L.
AU - Hamrick, Mark W
AU - Baile, Clifton A.
N1 - Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2006/2
Y1 - 2006/2
N2 - Genistein, an isoflavone in soybean products, has estrogenic activity and is used as a natural substitute for estrogen replacement therapy in postmenopausal women. Genistein was also shown to decrease fat pad weight in female mice. The primary objective of this study was to determine the effect of genistein on adipose tissue apoptosis in vitro and in vivo. 3T3-L1 preadipocytes and mature adipocytes were treated with 0, 1, 10, 100, and 400 μmol/L genistein and then assayed for apoptosis, whereas only mature adipocytes were assayed for viability. Mature adipocytes treated with genistein demonstrated a dose-related increase in apoptosis. Ovariectomized female mice (9 mo old) were given 0, 150, or 1500 mg/kg genistein in the semipurified phytoestrogen-free casein-based diet for 3 wk (n = 10). After mice were killed, body composition was determined by dual-energy X-ray absorptiometry analysis, and parametrial (PM), inguinal (ING), and retroperitoneal (RP) fat pads were weighed and assayed for apoptosis (% DNA fragmentation). Genistein (1500 mg/kg) reduced food intake (FI) by 14% (P < 0.01) and body weight (BW) by 9% (P < 0.01). Body composition was not significantly affected, but PM and ING weights were decreased 22% (P < 0.05) and 19% (P < 0.07), respectively, by 1500 mg/kg genistein. Apoptosis in ING fat was increased 290% (P < 0.05) by 1500 mg/kg genistein. These findings show that oral genistein treatment can reduce BW, mobilize body fat, and induce apoptosis of adipose tissue in ovariectomized female mice. Thus, genistein may be useful in treating or preventing increased adiposity after menopause.
AB - Genistein, an isoflavone in soybean products, has estrogenic activity and is used as a natural substitute for estrogen replacement therapy in postmenopausal women. Genistein was also shown to decrease fat pad weight in female mice. The primary objective of this study was to determine the effect of genistein on adipose tissue apoptosis in vitro and in vivo. 3T3-L1 preadipocytes and mature adipocytes were treated with 0, 1, 10, 100, and 400 μmol/L genistein and then assayed for apoptosis, whereas only mature adipocytes were assayed for viability. Mature adipocytes treated with genistein demonstrated a dose-related increase in apoptosis. Ovariectomized female mice (9 mo old) were given 0, 150, or 1500 mg/kg genistein in the semipurified phytoestrogen-free casein-based diet for 3 wk (n = 10). After mice were killed, body composition was determined by dual-energy X-ray absorptiometry analysis, and parametrial (PM), inguinal (ING), and retroperitoneal (RP) fat pads were weighed and assayed for apoptosis (% DNA fragmentation). Genistein (1500 mg/kg) reduced food intake (FI) by 14% (P < 0.01) and body weight (BW) by 9% (P < 0.01). Body composition was not significantly affected, but PM and ING weights were decreased 22% (P < 0.05) and 19% (P < 0.07), respectively, by 1500 mg/kg genistein. Apoptosis in ING fat was increased 290% (P < 0.05) by 1500 mg/kg genistein. These findings show that oral genistein treatment can reduce BW, mobilize body fat, and induce apoptosis of adipose tissue in ovariectomized female mice. Thus, genistein may be useful in treating or preventing increased adiposity after menopause.
KW - 3T3-L1 adipocytes
KW - Adipose tissue
KW - Apoptosis
KW - Isoflavone
KW - Weight loss
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U2 - 10.1093/jn/136.2.409
DO - 10.1093/jn/136.2.409
M3 - Article
C2 - 16424120
AN - SCOPUS:32444442906
SN - 0022-3166
VL - 136
SP - 409
EP - 414
JO - Journal of Nutrition
JF - Journal of Nutrition
IS - 2
ER -