Abstract
Protein tyrosine phosphorylation occurs as one of the earlier events in cancer cell-extracellular matrix (ECM) interaction. With immunoblot analysis and immunofluorescence microscopy, genistein was found to suppress the tyrosine phosphorylation of proteins located at the cell periphery, including a 125 kDa protein, when B16-BL6 melanoma cells attached to and interacted with ECM. When accompanied by the suppression of adhesion-induced protein tyrosine phosphorylation, the invasive potential of B16-BL6 cells through reconstituted basement membrane was decreased significantly. However, neither adhesive capability nor cell growth was significantly affected by genistein. Therefore, the interruption of cancer cell-ECM interaction by suppression of protein tyrosine phosphorylation may contribute to invasion prevention of genistein.
Original language | English (US) |
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Pages (from-to) | 117-124 |
Number of pages | 8 |
Journal | Cancer Letters |
Volume | 129 |
Issue number | 1 |
DOIs | |
State | Published - Jul 3 1998 |
Externally published | Yes |
Keywords
- Cell-extracellular matrix interaction
- Genistein
- Invasion
- Protein tyrosine phosphorylation
ASJC Scopus subject areas
- Oncology
- Cancer Research