TY - JOUR
T1 - Global analysis of small non-coding RNA populations across tissues in the malaria vector, anopheles gambiae
AU - Bryant, William Bart
AU - Ray, Savanna
AU - Mills, Mary Katherine
N1 - Funding Information:
Acknowledgments: We thank the University of Kansas Medical Center Genomics Core which is supported by NIH U54 HD 090216, COBRE 5P20GM104936-10, and NIH S10OD021743.
Funding Information:
Funding: This study was supported by R21AI139603 awarded to W.B.B. from NIH-NIAID. The sponsors had no input on design, interpretation or preparation of the manuscript.
Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2020/7
Y1 - 2020/7
N2 - Malaria is a major global health problem, where the anautogenous female mosquito Anopheles gambiae serves as a major vector. In order to combat this devastating disease, understanding mosquito physiology is paramount. Numerous studies in the vector field demonstrate that small non-coding RNAs (ncRNAs) play essential roles in numerous aspects of mosquito physiology. While our previous miRNA annotation work demonstrated expression dynamics across differing tissues, miRNAs represented less than 20% of all small ncRNAs in our small RNA-Seq libraries. To this end, we systematically classified multiple small ncRNA groups across mosquito tissues. Here we (i) determined a new enriched-midgut miRNA, (ii) updated the piRNA annotation in ovaries with a genomic map of unique-mapping piRNAs, (iii) identified pan-tissue and tissue-enriched mRNA-derived small ncRNAs, and (iv) assessed AGO1-and AGO2-loading of candidate small ncRNAs. Continued research will broaden our view of small ncRNAs and greatly aide in our understanding on how these molecules contribute to mosquito physiology.
AB - Malaria is a major global health problem, where the anautogenous female mosquito Anopheles gambiae serves as a major vector. In order to combat this devastating disease, understanding mosquito physiology is paramount. Numerous studies in the vector field demonstrate that small non-coding RNAs (ncRNAs) play essential roles in numerous aspects of mosquito physiology. While our previous miRNA annotation work demonstrated expression dynamics across differing tissues, miRNAs represented less than 20% of all small ncRNAs in our small RNA-Seq libraries. To this end, we systematically classified multiple small ncRNA groups across mosquito tissues. Here we (i) determined a new enriched-midgut miRNA, (ii) updated the piRNA annotation in ovaries with a genomic map of unique-mapping piRNAs, (iii) identified pan-tissue and tissue-enriched mRNA-derived small ncRNAs, and (iv) assessed AGO1-and AGO2-loading of candidate small ncRNAs. Continued research will broaden our view of small ncRNAs and greatly aide in our understanding on how these molecules contribute to mosquito physiology.
KW - Mosquito reproduction
KW - PiRNA
KW - Small RNAs
KW - Small non-coding RNA
UR - http://www.scopus.com/inward/record.url?scp=85087177306&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85087177306&partnerID=8YFLogxK
U2 - 10.3390/insects11070406
DO - 10.3390/insects11070406
M3 - Article
AN - SCOPUS:85087177306
SN - 2075-4450
VL - 11
SP - 1
EP - 14
JO - Insects
JF - Insects
IS - 7
M1 - 406
ER -
