Glutathione levels during thermal induction of the yeast-to-mycelial transition in Candida albicans

Donovan Thomas; Katherine Klein; Elias Manavathu; Jonathan R. Dimmock; Bulent Mutus

doi:10.1111/j.1574-6968.1991.tb04371.x

Glutathione levels during thermal induction of the yeast-to-mycelial transition in Candida albicans

Donovan Thomas
, Katherine Klein
, Elias Manavathu
, Jonathan R. Dimmock
, Bulent Mutus

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Glutathione (GSH) levels were directly monitored by reverse phase HPLC during the thermal yeast-to-mycelial induction of Candida albicans. The GSH levels decreased ∼ 100-fold within 120 min which corresponded to the time of maximal yeast-to-mold conversion. The yeast to mold conversion was inhibited by 1-p-chlorophenyl-4,4-dimethyl-5-diethylamino-1-penten-3-one (CDDP) [1], a thiol-specific alkylator, which prevented the decline in GSH levels. These results are discussed with respect to the potential involvement of intracellular GSH levels in regulation of the yeast-to-mold dimorphism in Candida albicans.

Original language	English (US)
Pages (from-to)	331-334
Number of pages	4
Journal	FEMS Microbiology Letters
Volume	77
Issue number	2-3
DOIs	https://doi.org/10.1111/j.1574-6968.1991.tb04371.x
State	Published - Jan 15 1991
Externally published	Yes

Keywords

Candida albicans
Glutathione
Yeast to mold transition

ASJC Scopus subject areas

General Medicine

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10.1111/j.1574-6968.1991.tb04371.x

Cite this

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title = "Glutathione levels during thermal induction of the yeast-to-mycelial transition in Candida albicans",

abstract = "Glutathione (GSH) levels were directly monitored by reverse phase HPLC during the thermal yeast-to-mycelial induction of Candida albicans. The GSH levels decreased ∼ 100-fold within 120 min which corresponded to the time of maximal yeast-to-mold conversion. The yeast to mold conversion was inhibited by 1-p-chlorophenyl-4,4-dimethyl-5-diethylamino-1-penten-3-one (CDDP) [1], a thiol-specific alkylator, which prevented the decline in GSH levels. These results are discussed with respect to the potential involvement of intracellular GSH levels in regulation of the yeast-to-mold dimorphism in Candida albicans.",

keywords = "Candida albicans, Glutathione, Yeast to mold transition",

author = "Donovan Thomas and Katherine Klein and Elias Manavathu and Dimmock, \{Jonathan R.\} and Bulent Mutus",

note = "Funding Information: This researchw as supportedb y grants from National Science and EngineeringR esearch Council of Canada.K .K. receiveda n Employment and ImmigrationC anadaS ummerE mployment/ ExperienceD evelopmenPt rogrammeg rant.The authors would like to thank Mr. C. Hogue for developingth e HPLC methodology.",

year = "1991",

month = jan,

day = "15",

doi = "10.1111/j.1574-6968.1991.tb04371.x",

language = "English (US)",

volume = "77",

pages = "331--334",

journal = "FEMS Microbiology Letters",

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publisher = "Oxford University Press",

number = "2-3",

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TY - JOUR

T1 - Glutathione levels during thermal induction of the yeast-to-mycelial transition in Candida albicans

AU - Thomas, Donovan

AU - Klein, Katherine

AU - Manavathu, Elias

AU - Dimmock, Jonathan R.

AU - Mutus, Bulent

N1 - Funding Information: This researchw as supportedb y grants from National Science and EngineeringR esearch Council of Canada.K .K. receiveda n Employment and ImmigrationC anadaS ummerE mployment/ ExperienceD evelopmenPt rogrammeg rant.The authors would like to thank Mr. C. Hogue for developingth e HPLC methodology.

PY - 1991/1/15

Y1 - 1991/1/15

N2 - Glutathione (GSH) levels were directly monitored by reverse phase HPLC during the thermal yeast-to-mycelial induction of Candida albicans. The GSH levels decreased ∼ 100-fold within 120 min which corresponded to the time of maximal yeast-to-mold conversion. The yeast to mold conversion was inhibited by 1-p-chlorophenyl-4,4-dimethyl-5-diethylamino-1-penten-3-one (CDDP) [1], a thiol-specific alkylator, which prevented the decline in GSH levels. These results are discussed with respect to the potential involvement of intracellular GSH levels in regulation of the yeast-to-mold dimorphism in Candida albicans.

AB - Glutathione (GSH) levels were directly monitored by reverse phase HPLC during the thermal yeast-to-mycelial induction of Candida albicans. The GSH levels decreased ∼ 100-fold within 120 min which corresponded to the time of maximal yeast-to-mold conversion. The yeast to mold conversion was inhibited by 1-p-chlorophenyl-4,4-dimethyl-5-diethylamino-1-penten-3-one (CDDP) [1], a thiol-specific alkylator, which prevented the decline in GSH levels. These results are discussed with respect to the potential involvement of intracellular GSH levels in regulation of the yeast-to-mold dimorphism in Candida albicans.

KW - Candida albicans

KW - Glutathione

KW - Yeast to mold transition

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UR - https://www.scopus.com/pages/publications/0026424280#tab=citedBy

U2 - 10.1111/j.1574-6968.1991.tb04371.x

DO - 10.1111/j.1574-6968.1991.tb04371.x

M3 - Article

C2 - 2037237

AN - SCOPUS:0026424280

SN - 0378-1097

VL - 77

SP - 331

EP - 334

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 2-3

ER -

Glutathione levels during thermal induction of the yeast-to-mycelial transition in Candida albicans

Abstract

Keywords

ASJC Scopus subject areas

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