Heterogeneity of immune cells in human atherosclerosis revealed by scRNA-Seq

Jenifer Vallejo, Clément Cochain, Alma Zernecke, Klaus Ley

Research output: Contribution to journalReview articlepeer-review

23 Scopus citations


Immune cells in atherosclerosis include T, B, natural killer (NK) and NKT cells, macrophages, monocytes, dendritic cells (DCs), neutrophils, and mast cells. Advances in single-cell RNA sequencing (sRNA-Seq) have refined our understanding of immune cell subsets. Four recent studies have used scRNA-Seq of immune cells in human atherosclerotic lesions and peripheral blood mononuclear cells (PBMCs), some including cell surface phenotypes revealed by oligonucleotide-tagged antibodies, which confirmed known and identified new immune cell subsets and identified genes significantly up-regulated in PBMCs from HIV+ subjects with atherosclerosis compared to PBMCs from matched HIV+ subjects without atherosclerosis. The ability of scRNA-Seq to identify cell types is greatly augmented by adding cell surface phenotype using antibody sequencing. In this review, we summarize the latest data obtained by scRNA-Seq on plaques and human PBMCs in human subjects with atherosclerosis.

Original languageEnglish (US)
Pages (from-to)2537-2543
Number of pages7
JournalCardiovascular Research
Issue number13
StatePublished - Nov 15 2021
Externally publishedYes


  • Antibodies
  • Atherosclerosis
  • Human
  • Transcriptomes
  • scRNA-Seq

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)


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