TY - JOUR
T1 - High-performance liquid chromatographic determination of amino acids and oligopeptides by pre-column fluorescence derivatization with 9-fluorenyl- methoxy carbonyl succinimide
AU - You, Jinmao
AU - Fan, Xingjun
AU - Wang, Hongen
AU - Wang, Guojun
AU - Su, Ji Xin
AU - Zhou, Changli
PY - 1998
Y1 - 1998
N2 - A sensitive high-performance chromatographic method for the detection of amino acids and oligopeptides with pre-column fluorescence derivatization has been developed. Glycine, glycylglycine, triglycine, glutathion, glutamic acid, and cysteine were separated on a reversed-phase C18 column with methanol-water-triethylamine as eluent; the derivatization and the chromatographic conditions were optimized. The six derivatives were eluted in 20 rain with good reproducibility. The relative standard deviations (n=6) at an analytical concentration of 2 x 10-6 M are less than 4.5%. The detection limits (signal-to-noise ratio=3) for the six derivatives are in the range of 15-46 fmol.
AB - A sensitive high-performance chromatographic method for the detection of amino acids and oligopeptides with pre-column fluorescence derivatization has been developed. Glycine, glycylglycine, triglycine, glutathion, glutamic acid, and cysteine were separated on a reversed-phase C18 column with methanol-water-triethylamine as eluent; the derivatization and the chromatographic conditions were optimized. The six derivatives were eluted in 20 rain with good reproducibility. The relative standard deviations (n=6) at an analytical concentration of 2 x 10-6 M are less than 4.5%. The detection limits (signal-to-noise ratio=3) for the six derivatives are in the range of 15-46 fmol.
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U2 - 10.1080/10826079808006611
DO - 10.1080/10826079808006611
M3 - Article
AN - SCOPUS:0031822402
SN - 1082-6076
VL - 21
SP - 2103
EP - 2115
JO - Journal of Liquid Chromatography and Related Technologies
JF - Journal of Liquid Chromatography and Related Technologies
IS - 14
ER -