TY - JOUR
T1 - High performance liquid chromatographic determination of N- nitrosoamines by pre-column fluorescence derivatization with acridone-N- acetyl chloride
AU - You, Jinmao
AU - Fan, Xinjun
AU - Lao, Wenjian
AU - Ou, Qingyu
AU - Zhu, Qingcun
PY - 1999/2
Y1 - 1999/2
N2 - A sensitive high performance liquid chromatographic method for the determination of N-nitrosoamines with pre-column fluorescence derivatization has been developed. N-nitrosoamines are first changed into secondary amines using denitrosation reagent, then react with acridone-N-acetyl chloride (ARC- Cl) to produce corresponding secondary amine derivatives, which exhibit a strong fluorescence. Maximum emission for ARC derivatives is 430 nm (λ(ex) 404 nm). The labelled derivatives are very stable, less than 4% decomposition occurs after heating at 40°C for 24 h. Fluorescence intensities of derivatives are higher in neutral and alkaline than in acidic solutions. This method, in conjunction with a multi-gradient program, offers a baseline resolution of the ARC derivatives from a linear acetonitrile gradient. Separation is carried out on a reverse phase C18 column. Derivatization and chromatographic conditions are optimized. The relative standard deviation (n = 6) at an analytical concentration of 10 pmol of each N-nitroamine is less than 4.5%. The detection limits at the fmol level. The method described is also suitable for analysis of other amino compounds in different biological samples.
AB - A sensitive high performance liquid chromatographic method for the determination of N-nitrosoamines with pre-column fluorescence derivatization has been developed. N-nitrosoamines are first changed into secondary amines using denitrosation reagent, then react with acridone-N-acetyl chloride (ARC- Cl) to produce corresponding secondary amine derivatives, which exhibit a strong fluorescence. Maximum emission for ARC derivatives is 430 nm (λ(ex) 404 nm). The labelled derivatives are very stable, less than 4% decomposition occurs after heating at 40°C for 24 h. Fluorescence intensities of derivatives are higher in neutral and alkaline than in acidic solutions. This method, in conjunction with a multi-gradient program, offers a baseline resolution of the ARC derivatives from a linear acetonitrile gradient. Separation is carried out on a reverse phase C18 column. Derivatization and chromatographic conditions are optimized. The relative standard deviation (n = 6) at an analytical concentration of 10 pmol of each N-nitroamine is less than 4.5%. The detection limits at the fmol level. The method described is also suitable for analysis of other amino compounds in different biological samples.
KW - Acridone-N-acetyl chloride (ARC-Cl)
KW - Column liquid chromatography
KW - Denitrosation
KW - Fluorescence derivatization
KW - N-nitroso compounds
UR - http://www.scopus.com/inward/record.url?scp=0032907220&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032907220&partnerID=8YFLogxK
U2 - 10.1016/S0039-9140(98)00265-3
DO - 10.1016/S0039-9140(98)00265-3
M3 - Article
C2 - 18967483
AN - SCOPUS:0032907220
SN - 0039-9140
VL - 48
SP - 437
EP - 449
JO - Talanta
JF - Talanta
IS - 2
ER -