Abstract
A highly sensitive HPLC method for the detection of amino acids and oligopeptides with 9-acridine formyl chloride by pre-column fluorescence derivatization has been developed. Glycine, glycylglycine, histidine, triglycine and glutathione were separated on a reversed-phase C 18 column with methanol-water-triethylamine eluent, derivatization and chromatographic conditions were optimized. The five derivatives were eluted in 28 min with a good reproducibility. Linear range of the calibration graph was 0.08-260 nmol/ml -1. The relative standard deviations(n=6) are < 5%. Detection limits (signal-to-noise ratio=3) for the five derivatives are 20-40 fmol.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 875-878 |
| Number of pages | 4 |
| Journal | Chinese Chemical Letters |
| Volume | 8 |
| Issue number | 10 |
| State | Published - Dec 1 1997 |
| Externally published | Yes |
ASJC Scopus subject areas
- Chemistry(all)