Human interstitial retinol-binding protein (IRBP): cloning, partial sequence, and chromosomal localization

Gregory I Liou; S. L. Fong; J. Gosden; P. van Tuinen; D. H. Ledbetter; S. Christie; D. Rout; S. Bhattacharya; R. G. Cook; Y. Li; C. Wang; C. D.B. Bridges

doi:10.1007/BF01534925

Human interstitial retinol-binding protein (IRBP): cloning, partial sequence, and chromosomal localization

Gregory I Liou, S. L. Fong, J. Gosden, P. van Tuinen, D. H. Ledbetter, S. Christie, D. Rout, S. Bhattacharya, R. G. Cook, Y. Li, C. Wang, C. D.B. Bridges

Graduate Studies

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Abstract

A cloned 2184-bp cDNA coding for human interstitial retinol-binding protein (IRBP) has been isolated and sequenced. The probe hybridized to a 5.2-kb poly(A) RNA from human retinas. Nineteen tryptic peptides (363 amino acids) sequenced and purified from bovine IRBP could be aligned with 86-88% homology to the translated sequence. Two segments approximately 200 amino acids long were found to have a 41% residue identity, suggesting an internal duplication event. This cloned cDNA was used to probe DNA samples from a panel of 29 rodent-human somatic cell hybrids, mapping the structural gene for IRBP to chromosome 10. In situ hybridization suggested a regional localization near the centromere (p11.2→q11.2), although a secondary site of hybridization at q24→25 was also observed.

Original language	English (US)
Pages (from-to)	315-323
Number of pages	9
Journal	Somatic Cell and Molecular Genetics
Volume	13
Issue number	4
DOIs	https://doi.org/10.1007/BF01534925
State	Published - Jul 1987

ASJC Scopus subject areas

Genetics
Cell Biology

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title = "Human interstitial retinol-binding protein (IRBP): cloning, partial sequence, and chromosomal localization",

abstract = "A cloned 2184-bp cDNA coding for human interstitial retinol-binding protein (IRBP) has been isolated and sequenced. The probe hybridized to a 5.2-kb poly(A) RNA from human retinas. Nineteen tryptic peptides (363 amino acids) sequenced and purified from bovine IRBP could be aligned with 86-88% homology to the translated sequence. Two segments approximately 200 amino acids long were found to have a 41% residue identity, suggesting an internal duplication event. This cloned cDNA was used to probe DNA samples from a panel of 29 rodent-human somatic cell hybrids, mapping the structural gene for IRBP to chromosome 10. In situ hybridization suggested a regional localization near the centromere (p11.2→q11.2), although a secondary site of hybridization at q24→25 was also observed.",

author = "Liou, {Gregory I} and Fong, {S. L.} and J. Gosden and {van Tuinen}, P. and Ledbetter, {D. H.} and S. Christie and D. Rout and S. Bhattacharya and Cook, {R. G.} and Y. Li and C. Wang and Bridges, {C. D.B.}",

year = "1987",

month = jul,

doi = "10.1007/BF01534925",

language = "English (US)",

volume = "13",

pages = "315--323",

journal = "Somatic Cell and Molecular Genetics",

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T1 - Human interstitial retinol-binding protein (IRBP)

T2 - cloning, partial sequence, and chromosomal localization

AU - Liou, Gregory I

AU - Fong, S. L.

AU - Gosden, J.

AU - van Tuinen, P.

AU - Ledbetter, D. H.

AU - Christie, S.

AU - Rout, D.

AU - Bhattacharya, S.

AU - Cook, R. G.

AU - Li, Y.

AU - Wang, C.

AU - Bridges, C. D.B.

PY - 1987/7

Y1 - 1987/7

N2 - A cloned 2184-bp cDNA coding for human interstitial retinol-binding protein (IRBP) has been isolated and sequenced. The probe hybridized to a 5.2-kb poly(A) RNA from human retinas. Nineteen tryptic peptides (363 amino acids) sequenced and purified from bovine IRBP could be aligned with 86-88% homology to the translated sequence. Two segments approximately 200 amino acids long were found to have a 41% residue identity, suggesting an internal duplication event. This cloned cDNA was used to probe DNA samples from a panel of 29 rodent-human somatic cell hybrids, mapping the structural gene for IRBP to chromosome 10. In situ hybridization suggested a regional localization near the centromere (p11.2→q11.2), although a secondary site of hybridization at q24→25 was also observed.

AB - A cloned 2184-bp cDNA coding for human interstitial retinol-binding protein (IRBP) has been isolated and sequenced. The probe hybridized to a 5.2-kb poly(A) RNA from human retinas. Nineteen tryptic peptides (363 amino acids) sequenced and purified from bovine IRBP could be aligned with 86-88% homology to the translated sequence. Two segments approximately 200 amino acids long were found to have a 41% residue identity, suggesting an internal duplication event. This cloned cDNA was used to probe DNA samples from a panel of 29 rodent-human somatic cell hybrids, mapping the structural gene for IRBP to chromosome 10. In situ hybridization suggested a regional localization near the centromere (p11.2→q11.2), although a secondary site of hybridization at q24→25 was also observed.

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ER -

Human interstitial retinol-binding protein (IRBP): cloning, partial sequence, and chromosomal localization

Abstract

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