Hyaluronic acid (HA)-Gd(III) and HA-Fe(III) microgels as MRI contrast enhancing agents

Nurettin Sahiner, Evrim Umut, Selin S. Suner, Mehtap Sahiner, Mustafa Culha, Ramesh S. Ayyala

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


Hyaluronic acid (HA) was crosslinked with Gd(III) and Fe(III) ions rendering physically crosslinked HA-metal(III) microgels as magnetic resonance imaging (MRI) enhancing contrast agents. These HA-Gd(III) and HA-Fe(III) microgels are injectable with size range, 50–5000 nm in water. The same isoelectric point, pH 1.2 ± 0.1, was measured for both microgels. HA-Gd(III) and HA-Fe(III) microgels are hemo-compatible biomaterials and can be safely used in intravascular applications up to 1000 μg/mL concentration. Furthermore, no significant toxicity was attained as 95 ± 8 and 81 ± 2% cell viability on L929 fibroblast cells at 100 μg/mL of HA-Gd(III) and HA-Fe(III) microgels were measured. Moreover, HA-Gd(III) microgels were found to afford significant contrast improvement capability in MRI with proton relaxivity, r1 = 2.11 mM−1 s−1, comparable with the values reported for Gd(III) labeled functionalized HA gel systems and commercial Gd based contrast agents.

Original languageEnglish (US)
Article number118873
Pages (from-to)118873
JournalCarbohydrate Polymers
StatePublished - Feb 1 2022
Externally publishedYes


  • Blood compatible
  • Fe(II) chelating
  • HA-Fe(III) microgel
  • HA-Gd(III) microgel
  • Hyaluronic acid (HA)
  • Magnetic resonance imaging (MRI)

ASJC Scopus subject areas

  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry


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