Identification and characterization of estrogen-like components in commercial resin-based dental restorative materials.

J. B. Lewis; F. A. Rueggeberg; C. A. Lapp; J. W. Ergle; G. S. Schuster

doi:10.1007/s007840050087

Identification and characterization of estrogen-like components in commercial resin-based dental restorative materials.

J. B. Lewis, F. A. Rueggeberg, C. A. Lapp, J. W. Ergle, G. S. Schuster

Restorative Sciences

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

Recently, resin-based dental restorative materials have been targeted as potential sources of xenoestrogens, specifically bisphenol A (BPA) and bisphenol A dimethacrylate (BAD), which could contribute to overall estrogen load and result in deleterious side effects. The present study used high-pressure liquid chromatography (HPLC) to analyze twenty-eight different commercially available dental resins for the presence of BPA and/or BAD. In addition, sublines of the MCF-7 human breast tumor cell line were cultured in the presence of eluates from eleven of the dental resins and assessed for proliferative responses using the sulforhodamine B assay. Only one resin, Delton II, had detectable levels of BPA or BAD that could be verified by Fourier transform infrared spectrometry. Likewise, eluates from Delton II were the only samples that elicited a significant proliferative response in two of the MCF-7 sublines tested. Therefore, we conclude that dental resins in general do not represent a significant source of BPA or BAD exposure.

Original language	English (US)
Pages (from-to)	107-113
Number of pages	7
Journal	Clinical oral investigations
Volume	3
Issue number	3
DOIs	https://doi.org/10.1007/s007840050087
State	Published - 1999

ASJC Scopus subject areas

General Dentistry

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title = "Identification and characterization of estrogen-like components in commercial resin-based dental restorative materials.",

abstract = "Recently, resin-based dental restorative materials have been targeted as potential sources of xenoestrogens, specifically bisphenol A (BPA) and bisphenol A dimethacrylate (BAD), which could contribute to overall estrogen load and result in deleterious side effects. The present study used high-pressure liquid chromatography (HPLC) to analyze twenty-eight different commercially available dental resins for the presence of BPA and/or BAD. In addition, sublines of the MCF-7 human breast tumor cell line were cultured in the presence of eluates from eleven of the dental resins and assessed for proliferative responses using the sulforhodamine B assay. Only one resin, Delton II, had detectable levels of BPA or BAD that could be verified by Fourier transform infrared spectrometry. Likewise, eluates from Delton II were the only samples that elicited a significant proliferative response in two of the MCF-7 sublines tested. Therefore, we conclude that dental resins in general do not represent a significant source of BPA or BAD exposure.",

author = "Lewis, {J. B.} and Rueggeberg, {F. A.} and Lapp, {C. A.} and Ergle, {J. W.} and Schuster, {G. S.}",

note = "Funding Information: Acknowledgements The authors wish to thank Ms. Noriko Akamatsu for excellent technical assistance, Dr. Carlos Sonnenschein for the gift of the BUS subline, and the MCG Biocompatibility Group for its support. This research was funded by a grant from the American Dental Association Health Foundation.",

year = "1999",

doi = "10.1007/s007840050087",

language = "English (US)",

volume = "3",

pages = "107--113",

journal = "Clinical oral investigations",

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AU - Lapp, C. A.

AU - Ergle, J. W.

AU - Schuster, G. S.

N1 - Funding Information: Acknowledgements The authors wish to thank Ms. Noriko Akamatsu for excellent technical assistance, Dr. Carlos Sonnenschein for the gift of the BUS subline, and the MCG Biocompatibility Group for its support. This research was funded by a grant from the American Dental Association Health Foundation.

PY - 1999

Y1 - 1999

N2 - Recently, resin-based dental restorative materials have been targeted as potential sources of xenoestrogens, specifically bisphenol A (BPA) and bisphenol A dimethacrylate (BAD), which could contribute to overall estrogen load and result in deleterious side effects. The present study used high-pressure liquid chromatography (HPLC) to analyze twenty-eight different commercially available dental resins for the presence of BPA and/or BAD. In addition, sublines of the MCF-7 human breast tumor cell line were cultured in the presence of eluates from eleven of the dental resins and assessed for proliferative responses using the sulforhodamine B assay. Only one resin, Delton II, had detectable levels of BPA or BAD that could be verified by Fourier transform infrared spectrometry. Likewise, eluates from Delton II were the only samples that elicited a significant proliferative response in two of the MCF-7 sublines tested. Therefore, we conclude that dental resins in general do not represent a significant source of BPA or BAD exposure.

AB - Recently, resin-based dental restorative materials have been targeted as potential sources of xenoestrogens, specifically bisphenol A (BPA) and bisphenol A dimethacrylate (BAD), which could contribute to overall estrogen load and result in deleterious side effects. The present study used high-pressure liquid chromatography (HPLC) to analyze twenty-eight different commercially available dental resins for the presence of BPA and/or BAD. In addition, sublines of the MCF-7 human breast tumor cell line were cultured in the presence of eluates from eleven of the dental resins and assessed for proliferative responses using the sulforhodamine B assay. Only one resin, Delton II, had detectable levels of BPA or BAD that could be verified by Fourier transform infrared spectrometry. Likewise, eluates from Delton II were the only samples that elicited a significant proliferative response in two of the MCF-7 sublines tested. Therefore, we conclude that dental resins in general do not represent a significant source of BPA or BAD exposure.

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Identification and characterization of estrogen-like components in commercial resin-based dental restorative materials.

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