TY - JOUR
T1 - Identification of a novel alternative splicing isoform of human amyloid precursor protein gene, APP639
AU - Tang, Ke
AU - Wang, Chen
AU - Shen, Chengyong
AU - Sheng, Shuli
AU - Ravid, Rivka
AU - Jing, Naihe
PY - 2003/7
Y1 - 2003/7
N2 - Senile plaques, neurofibrillary tangles and amyloid-laden cerebral vessels are characteristic features in the brains of individuals with Alzheimer's disease (AD). The principal component of amyloid in senile plaque and amyloid-laden cerebral vessels is β-amyloid (Aβ), a peptide proteolytically derived from a large amyloid precursor protein (APP). To date, several alternatively spliced human APP transcripts have been described. Here, we report the identification of a novel alternative splicing isoform of the human APP gene, APP639, which excludes exon 2 as well as exons 7 and 8. RT-PCR and Southern blot analysis show that APP639 mRNA is expressed in many human fetal tissues. In contrast, the APP639 transcript is hardly detected in the aged human cerebral cortex from both pathologically confirmed sporadic AD cases and nondemented controls. However, APP639 mRNA exists in the adult human liver. Western blot analysis shows that the protein product produced from the APP639 cDNA could be recognized by the APP antibody, and it does lack the exon 2 coding region. These results suggest that APP639, a novel alternative splicing isoform of human APP gene, does exist in human tissues in vivo.
AB - Senile plaques, neurofibrillary tangles and amyloid-laden cerebral vessels are characteristic features in the brains of individuals with Alzheimer's disease (AD). The principal component of amyloid in senile plaque and amyloid-laden cerebral vessels is β-amyloid (Aβ), a peptide proteolytically derived from a large amyloid precursor protein (APP). To date, several alternatively spliced human APP transcripts have been described. Here, we report the identification of a novel alternative splicing isoform of the human APP gene, APP639, which excludes exon 2 as well as exons 7 and 8. RT-PCR and Southern blot analysis show that APP639 mRNA is expressed in many human fetal tissues. In contrast, the APP639 transcript is hardly detected in the aged human cerebral cortex from both pathologically confirmed sporadic AD cases and nondemented controls. However, APP639 mRNA exists in the adult human liver. Western blot analysis shows that the protein product produced from the APP639 cDNA could be recognized by the APP antibody, and it does lack the exon 2 coding region. These results suggest that APP639, a novel alternative splicing isoform of human APP gene, does exist in human tissues in vivo.
KW - APP antibody
KW - Alternative splicing
KW - Alzheimer's disease
KW - Human
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U2 - 10.1046/j.1460-9568.2003.02731.x
DO - 10.1046/j.1460-9568.2003.02731.x
M3 - Article
C2 - 12859342
AN - SCOPUS:0042668370
SN - 0953-816X
VL - 18
SP - 102
EP - 108
JO - European Journal of Neuroscience
JF - European Journal of Neuroscience
IS - 1
ER -