Identification of quantum dot bioconjugates and cellular protein co-localization by hybrid gel blotting

Hong Yan Liu; Tania Q. Vu

doi:10.1021/nl070239e

Identification of quantum dot bioconjugates and cellular protein co-localization by hybrid gel blotting

Hong Yan Liu
, Tania Q. Vu

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

New approaches are needed to address the interaction of nanoparticles and cellular proteins at the molecular level. We present a modification of PAGE co-immunoprecipitation, QD-based PA-AGE electrophoresis blotting, and apply this to identify quantum dot (QD) bioconjugate-cellular protein association. This method provides the capability to isolate and evaluate the action of QD bioconjugate-protein complexes in intact cells and to correlate these identified interactions with their location in cells.

Original language	English (US)
Pages (from-to)	1044-1049
Number of pages	6
Journal	Nano Letters
Volume	7
Issue number	4
DOIs	https://doi.org/10.1021/nl070239e
State	Published - Apr 2007
Externally published	Yes

ASJC Scopus subject areas

Bioengineering
General Chemistry
General Materials Science
Condensed Matter Physics
Mechanical Engineering

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10.1021/nl070239e

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abstract = "New approaches are needed to address the interaction of nanoparticles and cellular proteins at the molecular level. We present a modification of PAGE co-immunoprecipitation, QD-based PA-AGE electrophoresis blotting, and apply this to identify quantum dot (QD) bioconjugate-cellular protein association. This method provides the capability to isolate and evaluate the action of QD bioconjugate-protein complexes in intact cells and to correlate these identified interactions with their location in cells.",

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AB - New approaches are needed to address the interaction of nanoparticles and cellular proteins at the molecular level. We present a modification of PAGE co-immunoprecipitation, QD-based PA-AGE electrophoresis blotting, and apply this to identify quantum dot (QD) bioconjugate-cellular protein association. This method provides the capability to isolate and evaluate the action of QD bioconjugate-protein complexes in intact cells and to correlate these identified interactions with their location in cells.

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JO - Nano Letters

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Identification of quantum dot bioconjugates and cellular protein co-localization by hybrid gel blotting

Abstract

ASJC Scopus subject areas

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