IL-10 supplementation increases Tregs and decreases hypertension in the RUPP rat model of preeclampsia

Ashlyn Harmon; Denise Cornelius; Lorena Amaral; Adrienne Paige; Florian Herse; Tarek Ibrahim; Gerd Wallukat; Jessica Faulkner; Janae Moseley; Ralf Dechend; Babbette LaMarca

doi:10.3109/10641955.2015.1032054

IL-10 supplementation increases Tregs and decreases hypertension in the RUPP rat model of preeclampsia

Ashlyn Harmon, Denise Cornelius, Lorena Amaral, Adrienne Paige, Florian Herse, Tarek Ibrahim, Gerd Wallukat, Jessica Faulkner, Janae Moseley, Ralf Dechend, Babbette LaMarca

Research output: Contribution to journal › Article › peer-review

62 Scopus citations

Abstract

Objective: The reduced uterine perfusion pressure (RUPP) rat model of preeclampsia was used to determine the effects of added interleukin-10 (IL-10) on Tregs and hypertension in response to placental ischemia and how the decrease in these anti-inflammatory factors mediates the pathophysiology of preeclampsia.Methods: IL-10 (2.5 ng/kg/d) was infused via osmotic mini-pump implanted intraperitoneally on day 14 of gestation and, at the same time, the RUPP procedure was performed.Results: IL-10 reduced mean arterial pressure (p < 0.001), decreased CD4⁺ T cells (p = 0.044), while increasing Tregs (p = 0.043) which led to lower IL-6 and TNF- (p = 0.008 and p = 0.003), reduced AT1-AA production (p < 0.001), and decreased oxidative stress (p = 0.029) in RUPP rats.Conclusion: These data indicate that IL-10 supplementation increases Tregs and helps to balance the altered immune system seen during preeclampsia.

Original language	English (US)
Pages (from-to)	291-306
Number of pages	16
Journal	Hypertension in Pregnancy
Volume	34
Issue number	3
DOIs	https://doi.org/10.3109/10641955.2015.1032054
State	Published - Jul 3 2015
Externally published	Yes

Keywords

Hypertension
inflammation
pregnancy

ASJC Scopus subject areas

Internal Medicine
Obstetrics and Gynecology

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10.3109/10641955.2015.1032054

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title = "IL-10 supplementation increases Tregs and decreases hypertension in the RUPP rat model of preeclampsia",

abstract = "Objective: The reduced uterine perfusion pressure (RUPP) rat model of preeclampsia was used to determine the effects of added interleukin-10 (IL-10) on Tregs and hypertension in response to placental ischemia and how the decrease in these anti-inflammatory factors mediates the pathophysiology of preeclampsia.Methods: IL-10 (2.5 ng/kg/d) was infused via osmotic mini-pump implanted intraperitoneally on day 14 of gestation and, at the same time, the RUPP procedure was performed.Results: IL-10 reduced mean arterial pressure (p < 0.001), decreased CD4+ T cells (p = 0.044), while increasing Tregs (p = 0.043) which led to lower IL-6 and TNF- (p = 0.008 and p = 0.003), reduced AT1-AA production (p < 0.001), and decreased oxidative stress (p = 0.029) in RUPP rats.Conclusion: These data indicate that IL-10 supplementation increases Tregs and helps to balance the altered immune system seen during preeclampsia.",

keywords = "Hypertension, inflammation, pregnancy",

author = "Ashlyn Harmon and Denise Cornelius and Lorena Amaral and Adrienne Paige and Florian Herse and Tarek Ibrahim and Gerd Wallukat and Jessica Faulkner and Janae Moseley and Ralf Dechend and Babbette LaMarca",

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doi = "10.3109/10641955.2015.1032054",

language = "English (US)",

volume = "34",

pages = "291--306",

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T1 - IL-10 supplementation increases Tregs and decreases hypertension in the RUPP rat model of preeclampsia

AU - Harmon, Ashlyn

AU - Cornelius, Denise

AU - Amaral, Lorena

AU - Paige, Adrienne

AU - Herse, Florian

AU - Ibrahim, Tarek

AU - Wallukat, Gerd

AU - Faulkner, Jessica

AU - Moseley, Janae

AU - Dechend, Ralf

AU - LaMarca, Babbette

PY - 2015/7/3

Y1 - 2015/7/3

N2 - Objective: The reduced uterine perfusion pressure (RUPP) rat model of preeclampsia was used to determine the effects of added interleukin-10 (IL-10) on Tregs and hypertension in response to placental ischemia and how the decrease in these anti-inflammatory factors mediates the pathophysiology of preeclampsia.Methods: IL-10 (2.5 ng/kg/d) was infused via osmotic mini-pump implanted intraperitoneally on day 14 of gestation and, at the same time, the RUPP procedure was performed.Results: IL-10 reduced mean arterial pressure (p < 0.001), decreased CD4+ T cells (p = 0.044), while increasing Tregs (p = 0.043) which led to lower IL-6 and TNF- (p = 0.008 and p = 0.003), reduced AT1-AA production (p < 0.001), and decreased oxidative stress (p = 0.029) in RUPP rats.Conclusion: These data indicate that IL-10 supplementation increases Tregs and helps to balance the altered immune system seen during preeclampsia.

AB - Objective: The reduced uterine perfusion pressure (RUPP) rat model of preeclampsia was used to determine the effects of added interleukin-10 (IL-10) on Tregs and hypertension in response to placental ischemia and how the decrease in these anti-inflammatory factors mediates the pathophysiology of preeclampsia.Methods: IL-10 (2.5 ng/kg/d) was infused via osmotic mini-pump implanted intraperitoneally on day 14 of gestation and, at the same time, the RUPP procedure was performed.Results: IL-10 reduced mean arterial pressure (p < 0.001), decreased CD4+ T cells (p = 0.044), while increasing Tregs (p = 0.043) which led to lower IL-6 and TNF- (p = 0.008 and p = 0.003), reduced AT1-AA production (p < 0.001), and decreased oxidative stress (p = 0.029) in RUPP rats.Conclusion: These data indicate that IL-10 supplementation increases Tregs and helps to balance the altered immune system seen during preeclampsia.

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KW - inflammation

KW - pregnancy

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IL-10 supplementation increases Tregs and decreases hypertension in the RUPP rat model of preeclampsia

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