Immunization with a LEAPS(TM) heteroconjugate containing a CTL epitope and a peptide from beta-2-microglobulin elicits a protective and DTH response to herpes simplex virus type 1

Ken S. Rosenthal; Hanwen Mao; Walter I. Horne; Craig Wright; Daniel Zimmerman

doi:10.1016/S0264-410X(98)00231-X

Immunization with a LEAPS(TM) heteroconjugate containing a CTL epitope and a peptide from beta-2-microglobulin elicits a protective and DTH response to herpes simplex virus type 1

Ken S. Rosenthal, Hanwen Mao, Walter I. Horne, Craig Wright, Daniel Zimmerman

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

A ligand epitope antigen presentation system (LEAPS(TM)) heteroconjugate vaccine containing a CTL epitope (H1) from the HSV-1 immediate early protein ICP27 (322-332) and a peptide sequence (J) from β-2-microglobulin (35-50) elicited protection from intraperitoneal viral challenge and promoted DTH responses. The H1 peptide and other H1 containing heteroconjugates did not elicit protection or DTH responses. Antibody to the H1 peptide could not be detected by ELISA following vaccination with peptide, heteroconjugate or natural infection. The LEAPS(TM) heteroconjugate appears to prime a Th1-like response which is subsequently boosted by infection. These studies show that attachment of the J peptide can make a CTL epitope into a vaccine which is immunogenic and promotes a protective Th1 type of response.

Original language	English (US)
Pages (from-to)	535-542
Number of pages	8
Journal	Vaccine
Volume	17
Issue number	6
DOIs	https://doi.org/10.1016/S0264-410X(98)00231-X
State	Published - Feb 1999
Externally published	Yes

Keywords

Herpes simplex virus
Heteroconjugate
Th1 response

ASJC Scopus subject areas

Molecular Medicine
Immunology and Microbiology(all)
veterinary(all)
Public Health, Environmental and Occupational Health
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0264-410X(98)00231-X

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title = "Immunization with a LEAPS(TM) heteroconjugate containing a CTL epitope and a peptide from beta-2-microglobulin elicits a protective and DTH response to herpes simplex virus type 1",

abstract = "A ligand epitope antigen presentation system (LEAPS(TM)) heteroconjugate vaccine containing a CTL epitope (H1) from the HSV-1 immediate early protein ICP27 (322-332) and a peptide sequence (J) from β-2-microglobulin (35-50) elicited protection from intraperitoneal viral challenge and promoted DTH responses. The H1 peptide and other H1 containing heteroconjugates did not elicit protection or DTH responses. Antibody to the H1 peptide could not be detected by ELISA following vaccination with peptide, heteroconjugate or natural infection. The LEAPS(TM) heteroconjugate appears to prime a Th1-like response which is subsequently boosted by infection. These studies show that attachment of the J peptide can make a CTL epitope into a vaccine which is immunogenic and promotes a protective Th1 type of response.",

keywords = "Herpes simplex virus, Heteroconjugate, Th1 response",

author = "Rosenthal, {Ken S.} and Hanwen Mao and Horne, {Walter I.} and Craig Wright and Daniel Zimmerman",

year = "1999",

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doi = "10.1016/S0264-410X(98)00231-X",

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T1 - Immunization with a LEAPS(TM) heteroconjugate containing a CTL epitope and a peptide from beta-2-microglobulin elicits a protective and DTH response to herpes simplex virus type 1

AU - Rosenthal, Ken S.

AU - Mao, Hanwen

AU - Horne, Walter I.

AU - Wright, Craig

AU - Zimmerman, Daniel

PY - 1999/2

Y1 - 1999/2

N2 - A ligand epitope antigen presentation system (LEAPS(TM)) heteroconjugate vaccine containing a CTL epitope (H1) from the HSV-1 immediate early protein ICP27 (322-332) and a peptide sequence (J) from β-2-microglobulin (35-50) elicited protection from intraperitoneal viral challenge and promoted DTH responses. The H1 peptide and other H1 containing heteroconjugates did not elicit protection or DTH responses. Antibody to the H1 peptide could not be detected by ELISA following vaccination with peptide, heteroconjugate or natural infection. The LEAPS(TM) heteroconjugate appears to prime a Th1-like response which is subsequently boosted by infection. These studies show that attachment of the J peptide can make a CTL epitope into a vaccine which is immunogenic and promotes a protective Th1 type of response.

AB - A ligand epitope antigen presentation system (LEAPS(TM)) heteroconjugate vaccine containing a CTL epitope (H1) from the HSV-1 immediate early protein ICP27 (322-332) and a peptide sequence (J) from β-2-microglobulin (35-50) elicited protection from intraperitoneal viral challenge and promoted DTH responses. The H1 peptide and other H1 containing heteroconjugates did not elicit protection or DTH responses. Antibody to the H1 peptide could not be detected by ELISA following vaccination with peptide, heteroconjugate or natural infection. The LEAPS(TM) heteroconjugate appears to prime a Th1-like response which is subsequently boosted by infection. These studies show that attachment of the J peptide can make a CTL epitope into a vaccine which is immunogenic and promotes a protective Th1 type of response.

KW - Herpes simplex virus

KW - Heteroconjugate

KW - Th1 response

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UR - http://www.scopus.com/inward/citedby.url?scp=0033547872&partnerID=8YFLogxK

U2 - 10.1016/S0264-410X(98)00231-X

DO - 10.1016/S0264-410X(98)00231-X

M3 - Article

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AN - SCOPUS:0033547872

SN - 0264-410X

VL - 17

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JO - Vaccine

JF - Vaccine

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ER -

Immunization with a LEAPS(TM) heteroconjugate containing a CTL epitope and a peptide from beta-2-microglobulin elicits a protective and DTH response to herpes simplex virus type 1

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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