Immunofluorescence analysis of the expression of Norpeg (Rai14) in retinal Müller and ganglion cells

R. Krishnan Kutty; William Samuel; Shanyi Chen; Camasamudram Vijayasarathy; Ying Dun; Barbara Mysona; Barbara Wiggert; Sylvia B. Smith

doi:10.1016/j.neulet.2006.06.006

Immunofluorescence analysis of the expression of Norpeg (Rai14) in retinal Müller and ganglion cells

R. Krishnan Kutty, William Samuel, Shanyi Chen, Camasamudram Vijayasarathy, Ying Dun, Barbara Mysona, Barbara Wiggert, Sylvia B. Smith

Cellular Biology and Anatomy

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Novel retinal pigment epithelial cell gene (Norpeg, Rai14), a developmentally regulated mouse gene, encodes a protein containing six ankyrin repeats and a coiled-coil domain. The objective of the present study was to characterize the Norpeg protein and to analyze its expression in mouse retina using an antibody preparation that we developed. The ∼110 kDa Norpeg was immunoprecipitated and characterized by mass spectrometry. Primary cultures of Müller and ganglion cells isolated from the mouse retina were found to express Norpeg when analyzed by immunoblotting and immunofluorescence microscopy. Immunofluorescence analysis of normal mouse retina sections demonstrated that Norpeg is expressed in cells of the ganglion cell layer, inner nuclear layer as well as in the retinal pigment epithelium. Immunoreactivity was also evident in the radial glial (Müller) cell fibers.

Original language	English (US)
Pages (from-to)	294-298
Number of pages	5
Journal	Neuroscience Letters
Volume	404
Issue number	3
DOIs	https://doi.org/10.1016/j.neulet.2006.06.006
State	Published - Sep 1 2006

Keywords

Ganglion cells
Immunofluorescence
Müller cells
Norpeg
Rai14
Retina

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/j.neulet.2006.06.006

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title = "Immunofluorescence analysis of the expression of Norpeg (Rai14) in retinal M{\"u}ller and ganglion cells",

abstract = "Novel retinal pigment epithelial cell gene (Norpeg, Rai14), a developmentally regulated mouse gene, encodes a protein containing six ankyrin repeats and a coiled-coil domain. The objective of the present study was to characterize the Norpeg protein and to analyze its expression in mouse retina using an antibody preparation that we developed. The ∼110 kDa Norpeg was immunoprecipitated and characterized by mass spectrometry. Primary cultures of M{\"u}ller and ganglion cells isolated from the mouse retina were found to express Norpeg when analyzed by immunoblotting and immunofluorescence microscopy. Immunofluorescence analysis of normal mouse retina sections demonstrated that Norpeg is expressed in cells of the ganglion cell layer, inner nuclear layer as well as in the retinal pigment epithelium. Immunoreactivity was also evident in the radial glial (M{\"u}ller) cell fibers.",

keywords = "Ganglion cells, Immunofluorescence, M{\"u}ller cells, Norpeg, Rai14, Retina",

author = "Kutty, {R. Krishnan} and William Samuel and Shanyi Chen and Camasamudram Vijayasarathy and Ying Dun and Barbara Mysona and Barbara Wiggert and Smith, {Sylvia B.}",

note = "Funding Information: We thank Ms. Leena Amarnath for expert technical assistance. This research was supported by the Intramural Research Program of the National Institutes of Health, National Eye Institute.",

year = "2006",

month = sep,

day = "1",

doi = "10.1016/j.neulet.2006.06.006",

language = "English (US)",

volume = "404",

pages = "294--298",

journal = "Neuroscience Letters",

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T1 - Immunofluorescence analysis of the expression of Norpeg (Rai14) in retinal Müller and ganglion cells

AU - Kutty, R. Krishnan

AU - Samuel, William

AU - Chen, Shanyi

AU - Vijayasarathy, Camasamudram

AU - Dun, Ying

AU - Mysona, Barbara

AU - Wiggert, Barbara

AU - Smith, Sylvia B.

N1 - Funding Information: We thank Ms. Leena Amarnath for expert technical assistance. This research was supported by the Intramural Research Program of the National Institutes of Health, National Eye Institute.

PY - 2006/9/1

Y1 - 2006/9/1

N2 - Novel retinal pigment epithelial cell gene (Norpeg, Rai14), a developmentally regulated mouse gene, encodes a protein containing six ankyrin repeats and a coiled-coil domain. The objective of the present study was to characterize the Norpeg protein and to analyze its expression in mouse retina using an antibody preparation that we developed. The ∼110 kDa Norpeg was immunoprecipitated and characterized by mass spectrometry. Primary cultures of Müller and ganglion cells isolated from the mouse retina were found to express Norpeg when analyzed by immunoblotting and immunofluorescence microscopy. Immunofluorescence analysis of normal mouse retina sections demonstrated that Norpeg is expressed in cells of the ganglion cell layer, inner nuclear layer as well as in the retinal pigment epithelium. Immunoreactivity was also evident in the radial glial (Müller) cell fibers.

AB - Novel retinal pigment epithelial cell gene (Norpeg, Rai14), a developmentally regulated mouse gene, encodes a protein containing six ankyrin repeats and a coiled-coil domain. The objective of the present study was to characterize the Norpeg protein and to analyze its expression in mouse retina using an antibody preparation that we developed. The ∼110 kDa Norpeg was immunoprecipitated and characterized by mass spectrometry. Primary cultures of Müller and ganglion cells isolated from the mouse retina were found to express Norpeg when analyzed by immunoblotting and immunofluorescence microscopy. Immunofluorescence analysis of normal mouse retina sections demonstrated that Norpeg is expressed in cells of the ganglion cell layer, inner nuclear layer as well as in the retinal pigment epithelium. Immunoreactivity was also evident in the radial glial (Müller) cell fibers.

KW - Ganglion cells

KW - Immunofluorescence

KW - Müller cells

KW - Norpeg

KW - Rai14

KW - Retina

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JO - Neuroscience Letters

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Immunofluorescence analysis of the expression of Norpeg (Rai14) in retinal Müller and ganglion cells

Abstract

Keywords

ASJC Scopus subject areas

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