TY - JOUR
T1 - Immunolocalization of podoplanin in benign odontogenic tumours with and without ectomesenchyme.
AU - Caetano, Adriana dos Santos
AU - Tjioe, Kellen Cristine
AU - Faustino, Simone Eloiza Sita
AU - Hanemann, João Adolfo Costa
AU - Belone, Andréa de Faria Fernandes
AU - Soares, Cléverson Teixeira
AU - Oliveira, Denise Tostes
N1 - Copyright © 2012 Elsevier Ltd. All rights reserved.
PY - 2012/7
Y1 - 2012/7
N2 - OBJECTIVES: To investigate podoplanin expression in epithelial odontogenic tumours with and without ectomesenchyme and verify the association between its immunoexpression and proliferative activity in keratocystic odontogenic tumours (KCOTS) and orthokeratinized odontogenic cysts (OOCs).DESIGN: Eight ameloblastomas, nine adenomatoid odontogenic tumours, twenty KCOTS, five OOC, one calcifying epithelial odontogenic tumour, two ameloblastic fibromas, four ameloblastic fibro-odontomas and five calcifying cystic odontogenic tumours were immunohistochemically analysed with anti-podoplanin antibody. For KCOTS and OOC, the cell proliferation index was determined with Ki-67 immunostaining and compared by Spearman correlation coefficient.RESULTS: Podoplanin was expressed in the peripheral odontogenic epithelium of most tumours. Ectomesenchyme was negative, except for odontoblasts. KCOTS exhibited positive podoplanin expression while in OOC it was absent/weak. There was statistically significant correlation (p=0.006) between podoplanin expression and cellular proliferation index of KCOTS and OOC.CONCLUSION: Podoplanin seems to be related to the proliferative activity of KCOTS and may have a role in the process of local invasion of odontogenic tumours with and without ectomesenchyme.
AB - OBJECTIVES: To investigate podoplanin expression in epithelial odontogenic tumours with and without ectomesenchyme and verify the association between its immunoexpression and proliferative activity in keratocystic odontogenic tumours (KCOTS) and orthokeratinized odontogenic cysts (OOCs).DESIGN: Eight ameloblastomas, nine adenomatoid odontogenic tumours, twenty KCOTS, five OOC, one calcifying epithelial odontogenic tumour, two ameloblastic fibromas, four ameloblastic fibro-odontomas and five calcifying cystic odontogenic tumours were immunohistochemically analysed with anti-podoplanin antibody. For KCOTS and OOC, the cell proliferation index was determined with Ki-67 immunostaining and compared by Spearman correlation coefficient.RESULTS: Podoplanin was expressed in the peripheral odontogenic epithelium of most tumours. Ectomesenchyme was negative, except for odontoblasts. KCOTS exhibited positive podoplanin expression while in OOC it was absent/weak. There was statistically significant correlation (p=0.006) between podoplanin expression and cellular proliferation index of KCOTS and OOC.CONCLUSION: Podoplanin seems to be related to the proliferative activity of KCOTS and may have a role in the process of local invasion of odontogenic tumours with and without ectomesenchyme.
KW - Biological Specimen Banks
KW - Cell Proliferation
KW - Epithelial Cells/metabolism
KW - Humans
KW - Immunohistochemistry
KW - Jaw Neoplasms/metabolism
KW - Ki-67 Antigen
KW - Membrane Glycoproteins/metabolism
KW - Mesoderm/metabolism
KW - Odontogenic Cysts/metabolism
KW - Odontogenic Tumors/metabolism
UR - http://europepmc.org/abstract/med/22763018
U2 - 10.1016/j.archoralbio.2012.06.002
DO - 10.1016/j.archoralbio.2012.06.002
M3 - Article
C2 - 22763018
SN - 0003-9969
VL - 58
SP - 408
EP - 415
JO - Archives of Oral Biology
JF - Archives of Oral Biology
IS - 4
ER -