TY - JOUR
T1 - Immunologic response of the laryngeal mucosa to extraesophageal reflux
AU - Birchall, Martin A.
AU - Bailey, Michael
AU - Gutowska-Owsiak, Danuta
AU - Johnston, Nikki
AU - Inman, Charlotte F.
AU - Stokes, Christopher R.
AU - Postma, Gregory
AU - Pazmany, Laszlo
AU - Koufman, Jamie A.
AU - Phillips, Anne
AU - Rees, Louisa E.
N1 - Funding Information:
From the Laryngeal Research Group (Birchall, Phillips, Rees) and the Faculty of Medical and Veterinary Sciences (Bailey, Inman, Stokes, Phillips, Rees), University of Bristol, Bristol, and the Division of Medicine, University of Liverpool, Liverpool (Gutowska-Owsiak, Pazmany), England, the Department of Otolaryngology and Communication Sciences, Medical College of Wisconsin, Milwaukee, Wisconsin (Johnston), the Center for Voice and Swallowing Disorders, Department of Otolaryngology, Medical College of Georgia, Augusta, Georgia (Postma), and the Voice Institute of New York, New York, New York (Koufman). Dr Rees was supported by a Courtenay-Cowlin Fellowship (University of Bristol), and Dr Birchall was supported by a Research Clinical Leave Fellowship from the Wellcome Trust.
PY - 2008/12
Y1 - 2008/12
N2 - Objectives: Extraesophageal reflux is common. The treatment costs are high, and there are associations with other diseases, including laryngeal cancer. Our studies of the mucosal immune response to this common inflammatory disease suggest an important role for the nonclassic antigen-presenting molecule CD1d in the response to inflammation. This study was performed to further explore the relationship between the CD1d-NKT cell-iGb3 axis and reflux. Methods: We carried out a prospective study of laryngeal biopsies from 12 patients with laryngopharyngeal reflux and 11 controls. Quantitative multiple-color immunofluorescence using antibodies for lymphocytes (CD3, CD161) and classic and nonclassic major histocompatibility complex (I, II, β2m, CD1d) was performed, and univariate and multivariate analysis and co-localization measurements were applied. Results: Epithelial major histocompatibility complex class I and II expression was unchanged by reflux, but expression of CD1d increased (p < 0.05; luminal layers) and confidence intervals diminished in the reflux group. Co-localization of NKT cells with CD1d increased in patients (p < 0.01); iGb3 exhibited strong expression throughout all layers of the laryngeal epithelium. Conclusions: These data indicate a role for the CD1d-NKT cell-iGb3 axis in response to extraesophageal reflux in humans. This represents a useful target for novel diagnostics and treatments for this common condition.
AB - Objectives: Extraesophageal reflux is common. The treatment costs are high, and there are associations with other diseases, including laryngeal cancer. Our studies of the mucosal immune response to this common inflammatory disease suggest an important role for the nonclassic antigen-presenting molecule CD1d in the response to inflammation. This study was performed to further explore the relationship between the CD1d-NKT cell-iGb3 axis and reflux. Methods: We carried out a prospective study of laryngeal biopsies from 12 patients with laryngopharyngeal reflux and 11 controls. Quantitative multiple-color immunofluorescence using antibodies for lymphocytes (CD3, CD161) and classic and nonclassic major histocompatibility complex (I, II, β2m, CD1d) was performed, and univariate and multivariate analysis and co-localization measurements were applied. Results: Epithelial major histocompatibility complex class I and II expression was unchanged by reflux, but expression of CD1d increased (p < 0.05; luminal layers) and confidence intervals diminished in the reflux group. Co-localization of NKT cells with CD1d increased in patients (p < 0.01); iGb3 exhibited strong expression throughout all layers of the laryngeal epithelium. Conclusions: These data indicate a role for the CD1d-NKT cell-iGb3 axis in response to extraesophageal reflux in humans. This represents a useful target for novel diagnostics and treatments for this common condition.
KW - CD1d
KW - Extraesophageal reflux
KW - NKT cell
KW - iGb3
UR - http://www.scopus.com/inward/record.url?scp=59649120967&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=59649120967&partnerID=8YFLogxK
U2 - 10.1177/000348940811701205
DO - 10.1177/000348940811701205
M3 - Article
C2 - 19140534
AN - SCOPUS:59649120967
SN - 0003-4894
VL - 117
SP - 891
EP - 895
JO - Annals of Otology, Rhinology and Laryngology
JF - Annals of Otology, Rhinology and Laryngology
IS - 12
ER -