Importance of E-selectin for firm leukocyte adhesion in vivo

Klaus Ley, Margaretta Allietta, Daniel C. Bullard, Sharon Morgan

Research output: Contribution to journalArticlepeer-review

40 Scopus citations


Leukocyte adhesion under flow is preferentially mediated by the selectins. In this study we used intravital microscopy to investigate whether E-selectin may promote firm leukocyte adhesion in vivo. E-Selectin is expressed by endothelial cells activated with tumor necrosis factor-α (TNF- α) and causes slow leukocyte rolling. Microinjection of formyl-methionyl- leucyl-phenylalanine (fMLP) or macrophage inflammatory protein-2 (MIP-2) next to a venule of the TNF-α-treated mouse cremaster muscle significantly increased the number of adherent leukocytes. In gene-targeted mice homozygous for a null mutation in the E-selectin gene or in wild-type mice treated with an E-selectin monoclonal antibody (mAb), this response was significantly attenuated (by >80%). No such defect was seen in intercellular adhesion molecule-1 (ICAM-1)-deficient mice. E-Selectin-null mice showed more rapid leukocyte rolling than wild-type or ICAM-l-deficient mice, resulting in significantly shortened leukocyte transit times through venules. Topical application of fMLP onto the whole cremaster muscle generated the same number of adherent leukocytes in wild-type and E-selectin-deficient mice. We conclude that slow leukocyte rolling through E-selectin results in long transit times, which are essential for efficient leukocyte adhesion in response to a local chemotactic stimulus.

Original languageEnglish (US)
Pages (from-to)287-294
Number of pages8
JournalCirculation research
Issue number3
StatePublished - Aug 10 1998
Externally publishedYes


  • E-selectin
  • ICAM-1
  • Leukocyte adhesion
  • Leukocyte transit
  • Rolling

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine


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